Doxycycline and Zinc Loaded Silica-Nanofibrous Polymers as Biomaterials for Bone Regeneration
Metadatos
Afficher la notice complèteAuteur
Toledano Pérez, Manuel; Toledano Osorio, Manuel; Osorio Ruiz, Raquel; Carrasco Carmona, ÁlvaroEditorial
MDPI
Materia
Bone regeneration Vascularization Macrophages Zinc Doxycycline non-resorbable polymer Cells Silica
Date
2020-05Referencia bibliográfica
Toledano, M., Toledano-Osorio, M., Osorio, R., Carrasco-Carmona, Á., Gutiérrez-Pérez, J. L., Gutiérrez-Corrales, A., ... & Torres-Lagares, D. (2020). Doxycycline and Zinc Loaded Silica-Nanofibrous Polymers as Biomaterials for Bone Regeneration. Polymers, 12(5), 1201. [doi:10.3390/polym12051201]
Patrocinador
Ministry of Economy and Competitiveness; European Union (EU) MINECO/FEDER MAT2017-85999PRésumé
Themain target of bone tissue engineeringis to design biomaterials that support bone regeneration
and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with
5% wt of SiO2-nanoparticles (HOOC-Si-Membrane) were doped with zinc (Zn-HOOC-Si-Membrane)
or doxycycline (Dox-HOOC-Si-Membrane). Critical bone defects were effectuated on six New
Zealand-bred rabbit skulls and covered with the membranes. After six weeks, the bone architecture
was evaluated with micro computed tomography. Three histological analyses were utilized to
analyse bone regeneration, including von Kossa silver nitrate, toluidine blue and fluorescence.
All membrane-treated defects exhibited higher number of osteocytes and bone perimeter than
the control group without the membrane. Zn-HOOC-Si-Membranes induced higher new bone
and osteoid area than those treated with HOOC-Si-Membranes, and control group, respectively.
Zn-HOOC-Si-Membranes and Dox-HOOC-Si-Membranes attained the lowest ratio M1 macrophages/M2
macrophages. Dox-HOOC-Si-Membranes caused the lowest number of osteoclasts, and bone density.
At the trabecular new bone, Zn-HOOC-Si-Membranes produced the highest angiogenesis, bone thickness,
connectivity, junctions and branches. Zn-HOOC-Si-Membranes enhanced biological activity, attained a
balanced remodeling, and achieved the greatest regenerative efficiency after osteogenesis and angiogenesis
assessments. The bone-integrated Zn-HOOC-Si-Membranes can be considered as bioactive modulators
provoking a M2 macrophages (pro-healing cells) increase, being a potential biomaterial for promoting
bone repair.