Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)
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MDPI
Materia
Pod maturation Transcription factors Photosynthesis Metabolism Phaseolus vulgaris
Date
2020-04Referencia bibliográfica
Gómez-Martín, C., Capel, C., González, A. M., Lebrón, R., Yuste-Lisbona, F. J., Hackenberg, M., ... & Lozano, R. (2020). Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.). Plants, 9(4), 545. [doi:10.3390/plants9040545]
Sponsorship
Ministerio de Ciencia, Innovacion y Universidades (MCIU/AEI/FEDER, UE) AGL2017-88174-R RTC2017-6198-2 AGL2017-88702-C2-2-R; Junta de Andalucia 6128Abstract
Pod maturation of common bean relies upon complex gene expression changes, which in
turn are crucial for seed formation and dispersal. Hence, dissecting the transcriptional regulation of
pod maturation would be of great significance for breeding programs. In this study, a comprehensive
characterization of expression changes has been performed in two common bean cultivars (ancient
and modern) by analyzing the transcriptomes of five developmental pod stages, from fruit setting to
maturation. RNA-seq analysis allowed for the identification of key genes shared by both accessions,
which in turn were homologous to known Arabidopsis maturation genes and furthermore showed
a similar expression pattern along the maturation process. Gene- expression changes suggested a role
in promoting an accelerated breakdown of photosynthetic and ribosomal machinery associated with
chlorophyll degradation and early activation of alpha-linolenic acid metabolism. A further study of
transcription factors and their DNA binding sites revealed three candidate genes whose functions
may play a dominant role in regulating pod maturation. Altogether, this research identifies the
first maturation gene set reported in common bean so far and contributes to a better understanding
of the dynamic mechanisms of pod maturation, providing potentially useful information for
genomic-assisted breeding of common bean yield and pod quality attributes.