Satellitome landscape analysis of Megaleporinus macrocephalus (Teleostei, Anostomidae) reveals intense accumulation of satellite sequences on the heteromorphic sex chromosome
Metadatos
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Springer Nature
Fecha
2019-04-10Referencia bibliográfica
Utsunomia, R., de Andrade Silva, D. M. Z., Ruiz-Ruano, F. J., Goes, C. A. G., Melo, S., Ramos, L. P., ... & Hashimoto, D. T. (2019). Satellitome landscape analysis of Megaleporinus macrocephalus (Teleostei, Anostomidae) reveals intense accumulation of satellite sequences on the heteromorphic sex chromosome. Scientific reports, 9(1), 1-10.
Resumen
The accumulation of repetitive DNA sequences on the sex-limited W or Y chromosomes is a well-known
process that is likely triggered by the suppression of recombination between the sex chromosomes,
which leads to major differences in their sizes and genetic content. Here, we report an analysis
conducted on the satellitome of Megaleporinus macrocephalus that focuses specifically on the satDNAs
that have been shown to have higher abundances in females and are putatively located on the W
chromosome in this species. We characterized 164 satellite families in M. macrocephalus, which is, by
far, the most satellite-rich species discovered to date. Subsequently, we mapped 30 satellites, 22 of
which were located on the W chromosome, and 14 were shown to exist only on the W chromosome.
Finally, we report two simple, quick and reliable methods that can be used for sex identification in M.
macrocephalus individuals using fin clips or scales, which could be applicable to future studies conducted
in the field of aquaculture.