Full Transcriptomic Response of Pseudomonas aeruginosa to an Inulin-Derived Fructooligosaccharide
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Rubio-Gómez, José Manuel; Molina Santiago, Carlos Alberto; Udaondo Domínguez, Zulema; Tena Garitaonaindia, Mireia; Krell, Tino; Ramos, Juan-Luis; Daddaoua, AbdelaliEditorial
Frontiers Media
Materia
RNA sequencing rt-qPCR Adhesion Developmental process Molecular transducer Pathogenicity
Date
2020-02-20Referencia bibliográfica
Rubio-Gómez JM, Santiago CM, Udaondo Z, Garitaonaindia MT, Krell T, Ramos J-L and Daddaoua A (2020) Full Transcriptomic Response of Pseudomonas aeruginosa to an Inulin-Derived Fructooligosaccharide. Front. Microbiol. 11:202. [doi: 10.3389/fmicb.2020.00202]
Sponsorship
This work was supported by grants from the Spanish Ministry for Economy and Competitiveness (AGL2017-85270-R). CS is funded by the program Juan de la Cierva-Formación (FJCI-2015-23810).Abstract
Pseudomonas aeruginosa is an ubiquitous gram-negative opportunistic human
pathogen which is not considered part of the human commensal gut microbiota.
However, depletion of the intestinal microbiota (Dysbiosis) following antibiotic treatment
facilitates the colonization of the intestinal tract by Multidrug-Resistant P. aeruginosa.
One possible strategy is based on the use of functional foods with prebiotic activity. The
bifidogenic effect of the prebiotic inulin and its hydrolyzed form (fructooligosaccharide:
FOS) is well established since they promote the growth of specific beneficial (probiotic)
gut bacteria such as bifidobacteria. Previous studies of the opportunistic nosocomial
pathogen Pseudomonas aeruginosa PAO1 have shown that inulin and to a greater
extent FOS reduce growth and biofilm formation, which was found to be due to a
decrease in motility and exotoxin secretion. However, the transcriptional basis for these
phenotypic alterations remains unclear. To address this question we conducted RNAsequence
analysis. Changes in the transcript level induced by inulin and FOS were
similar, but a set of transcript levels were increased in response to inulin and reduced
in the presence of FOS. In the presence of inulin or FOS, 260 and 217 transcript
levels, respectively, were altered compared to the control to which no polysaccharide
was added. Importantly, changes in transcript levels of 57 and 83 genes were found
to be specific for either inulin or FOS, respectively, indicating that both compounds
trigger different changes. Gene pathway analyses of differentially expressed genes
(DEG) revealed a specific FOS-mediated reduction in transcript levels of genes that
participate in several canonical pathways involved in metabolism and growth, motility,
biofilm formation, b-lactamase resistance, and in the modulation of type III and VI
secretion systems; results that have been partially verified by real time quantitative
PCR measurements. Moreover, we have identified a genomic island formed by a cluster of 15 genes, encoding uncharacterized proteins, which were repressed in the
presence of FOS. The analysis of isogenic mutants has shown that genes of this
genomic island encode proteins involved in growth, biofilm formation and motility. These
results indicate that FOS selectively modulates bacterial pathogenicity by interfering with
different signaling pathways.