Detection and variability analyses of CRISPR-like loci in the H. pylori genome
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García Zea, Jerson Alexander; Herrán Moreno, Roberto De La; Robles Rodríguez, Francisca; Navajas Pérez, Rafael; Ruiz Rejón, CarmeloEditorial
PeerJ
Materia
Variability CRISPR-like VlpC gene Phylogenetic marker Helicobacter pylori
Date
2019-01-11Referencia bibliográfica
García-Zea JA, de la Herrán R, Robles Rodríguez F, Navajas-Pérez R, Ruiz Rejón C. 2019. Detection and variability analyses of CRISPR-like loci in the H. pylori genome. PeerJ 7:e6221
Abstract
Helicobacter pylori is a human pathogenic bacterium with a high genomic plasticity.
Although the functional CRISPR-Cas system has not been found in its genome,
CRISPR-like loci have been recently identified. In this work, 53 genomes from different
geographical areas are analyzed for the search and analysis of variability of this type of
structure. We confirm the presence of a locus that was previously described in the
VlpC gene in al lgenomes, and we characterize new CRISPR-like loci in other genomic
locations. By studying the variability and gene location of these loci, the evolution and
the possible roles of these sequences are discussed. Additionally, the usefulness of this
type of sequences as a phylogenetic marker has been demonstrated, associating the
different strains by geographical area.