dc.contributor.author | Capitán Vallvey, Luis Fermín | |
dc.contributor.author | Valencia Mirón, María del Carmen | |
dc.contributor.author | Arana Nicolás, Evaristo de | |
dc.date.accessioned | 2014-07-08T11:45:49Z | |
dc.date.available | 2014-07-08T11:45:49Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Capitán-Vallvey, L.F.; Valencia, M.C.; Arana Nicolás, E. Flow-through spectrophotometric sensor for the determination of aspartame in low-calorie and dietary products. Analytical Sciences, 20(10): 1437-1442 (2004). [http://hdl.handle.net/10481/32481] | es_ES |
dc.identifier.issn | 0910-6340 | |
dc.identifier.uri | http://hdl.handle.net/10481/32481 | |
dc.description.abstract | A very simple flow-through sensor is presented for the determination of the intense sweetener aspartame in low-calorie and dietary products. The sensor is implemented in a monochannel flow-injection system with UV spectrophotometric detection using a Sephadex CM-C25 cationic exchanger packed 20 mm high in a flow cell. This method is based on the transient retention of a cationic species of the sweetener on the solid phase when a pH 5.0 acetic acid sodium acetate buffer (0.01 M) is used as a carrier (2.6 mL-1 min). The carrier itself elutes the analyte from the solid support, regenerating a sensing zone. Aspartame was determined by measuring its intrinsic absorbance at 219 nm at its residence time without any derivatization. Calibration graphs were linear over the range of 5.0 - 600.0 µg mL-1 with an RSD of 0.55% (peak height). This sweetener was determined in several samples by measuring the height or peak area, obtaining recoveries ranging between 95 - 101% and 97.5 - 101%, respectively. The procedure was validated for its use in the determination of aspartame in low-calorie and dietary products, giving reproducible and accurate results. | es_ES |
dc.description.sponsorship | Financial support from the Ministerio de Educación y Cultura, Dirección General de Enseñanza Superior (Spain), project n. BQU2002-00353. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Japan Society for Analytical Chemistry (JSAC) | es_ES |
dc.subject | Chromatography mass-spectrometry | es_ES |
dc.subject | Capillary electrophoresis | es_ES |
dc.subject | Artificial sweeteners | es_ES |
dc.subject | Amino acids | es_ES |
dc.subject | Soft drinks | es_ES |
dc.subject | Ninhydrin | es_ES |
dc.subject | Electrode | es_ES |
dc.subject | Reagent | es_ES |
dc.title | Flow-through spectrophotometric sensor for the determination of aspartame in low-calorie and dietary products | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |