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Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals
dc.contributor.author | Andersen, Helle Raun | |
dc.contributor.author | Andersson, Anna-Maria | |
dc.contributor.author | Arnold, Steven F. | |
dc.contributor.author | Autrup, Herman | |
dc.contributor.author | Barfoed, Marianne | |
dc.contributor.author | Beresford, Nicola | |
dc.contributor.author | Bjerregaard, Poul | |
dc.contributor.author | Christiansen, Lisette | |
dc.contributor.author | Gissel, Birgitte | |
dc.contributor.author | Hummel, René | |
dc.contributor.author | Jørgensen, Eva B. | |
dc.contributor.author | Korsgaard, Bodil | |
dc.contributor.author | Guevel, Remy Le | |
dc.contributor.author | Leffers, Henrik | |
dc.contributor.author | McLachlan, John A. | |
dc.contributor.author | Møller, Anette | |
dc.contributor.author | Nielsen, Jesper Bo | |
dc.contributor.author | Olea Serrano, Nicolás | |
dc.contributor.author | Oles-Karasko, Anita | |
dc.contributor.author | Pakdel, Farzad | |
dc.contributor.author | Pedersen, Knud L. | |
dc.contributor.author | Pérez, Pilar | |
dc.contributor.author | Skakkebæk, Niels | |
dc.contributor.author | Sonnenschein, Carlos | |
dc.contributor.author | Soto, Ana M. | |
dc.contributor.author | Sumpter, John P. | |
dc.contributor.author | Thorpe, Susan | |
dc.contributor.author | Grandjean, Philippe | |
dc.date.accessioned | 2013-05-08T13:13:07Z | |
dc.date.available | 2013-05-08T13:13:07Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | Andersen, H.R.; et al. Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals. Environmental Health Perspectives, 107(sup.1): 89-108 (1999). [http://hdl.handle.net/10481/24990] | es_ES |
dc.identifier.issn | 0091-6765 | |
dc.identifier.issn | 1552-9924 | |
dc.identifier.other | PMCID: PMC1566352 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/24990 | |
dc.description.abstract | The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods. | es_ES |
dc.description.sponsorship | This study was supported by grants from the European Commission (Biomedicine and Health Research and Technological Programme, BMH4-CT96-03 14), the Danish Environmental Research Programme (96.01.015.16), and the Danish Medical Research Council (9401656). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | National Institute of Environmental Health Sciences | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | Estrogenic chemicals | es_ES |
dc.subject | Estrogens | es_ES |
dc.subject | Antiestrogens | es_ES |
dc.subject | Estrogenicity tests | es_ES |
dc.subject | Binding assay | es_ES |
dc.subject | Yeast | es_ES |
dc.subject | MCF-7 | es_ES |
dc.subject | Vitellogenin | es_ES |
dc.title | Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |