Enhancing Drought Tolerance in Lettuce: The Efficacy of the Seaweed-Derived Biostimulant Cytolan® Stress Applied at Different Growth Stages

Abstract

Water stress is one of the foremost global abiotic stressors limiting agricultural productivity. Biostimulants and bioactive compounds are emerging as promising tools to enhance crop stress tolerance. This study investigates the effects of Cytolan® Stress, a novel seaweed-derived biostimulant, on the water stress tolerance of lettuce plants. Three application strategies were evaluated: priming, where the biostimulant is applied before the onset of stress to prepare the plants for adverse conditions; buffering, involving application at the onset of stress to mitigate its immediate effects; and detoxifying, where the biostimulant is applied after stress to aid in plant recovery. Biomass, stress-related parameters, antioxidant activity, osmoprotectant levels, and photosynthesis-related metrics were analyzed to elucidate its potential mechanisms of action. The results demonstrated that Cytolan® Stress in priming and buffering applications significantly improved water stress tolerance, reducing biomass loss from 45% to only 25%. Moreover, the detoxifying treatment was the most effective, as plants showed biomass values similar to those of the control plants. The biostimulant reduced oxidative stress indicators while enhancing antioxidant defenses, including ascorbate (AsA)-glutathione (GSH) cycle, antioxidant compounds, and enzyme activities. In addition, Cytolan® Stress preserved photosynthesis performance under water stress conditions. These findings highlight the potential of Cytolan® Stress to mitigate drought stress effects in lettuce, offering broader implications for crop tolerance and resilience under water-limited conditions. Further studies are recommended to explore its efficacy across different crops and stress scenarios.