Changes in structure and performance during diafiltration of binary protein solutions due to repeated cycles of fouling/alkaline cleaning

Abstract

The purpose of this work was to evaluate the effect of the temperature (50 and 60 °C) of a NaOH cleaning solution during the diafiltration of a binary mixture of bovine serum albumin and β-lactoglobulin, through a 300 kDa tubular ceramic membrane along repeated operational cycles. To this aim, final permeate volume, membrane and fouling resistances and individual protein concentration were analyzed. At the end of each individual study, the membranes were characterized by liquid–liquid displacement porosimetry. As a result, 50 °C was found to be the most appropriated temperature due to its higher capability to restore the initial membrane resistance and the higher efficiency achieved in terms of protein separation. Both conditions fulfilled without altering the structural properties of the membrane as given by porosimetric analysis. In contrast, a great fouling resistance involving null protein transmission occurred when cleaning was performed at 60 °C.