@misc{10481/90173,
year = {2019},
month = {9},
url = {https://hdl.handle.net/10481/90173},
abstract = {Scleral defects can result as a consequence of trauma, infectious diseases or cancer and surgical repair with
allogeneic scleral grafts can be required. However, this method has limitations and novel alternatives are
needed. Here, the efficacy of acellular nanostructured fibrin-agarose hydrogel-based substitutes (NFAH) in the
repair of scleral defects in rabbits was studied. For this, scleral defects of 5-mm diameter were made on 18
adult-male New Zealand rabbits and repaired with acellular NFAH, NFAH crosslinked with genipin (NFAH-GP) or
glutaraldehyde (NFAH-GA), allogeneic scleral grafts as control (C-CTR) or not repaired (negative control N-CTR)
(n=3 each). Macroscopic and histological analyses were performed after 40-days. Macroscopy confirmed the
repair of all defects in a comparable manner than the C-CTR. Histology showed no degradation nor integration in
C-CTR while NFAH-GP and NFAH-GA biomaterials were encapsulated by connective and inflammatory tissues
with partial biodegradation. The NFAH were fully biodegraded and replaced by a loose connective tissue and
sclera covering the defects. This in vivo study demonstrated that the NFAH are a promising biocompatible and
pro-regenerative alternative to the use of allogeneic cadaveric grafts. However, large defects and long-term
studies are needed to demonstrate the potential clinical usefulness of these substitutes.},
organization = {Junta de Andalucía  CS PI-0400-2016},
organization = {Ministerio de Economía y Competitividad (Instituto de Salud Carlos III) FIS PI17/391},
organization = {“Fondo Europeo de Desarrollo Regional (FEDER),” European Union},
publisher = {Elsevier},
keywords = {Escleral surgical repair},
keywords = {Hydrogels},
keywords = {Crosslinking},
keywords = {Tissue engineering},
keywords = {Eyeball},
keywords = {Fibrin/agarose},
keywords = {Histology},
title = {Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits.},
doi = {10.1016/j.exer.2019.107717},
author = {Carriel Araya, Víctor and Vizcaíno López, Gerson and Chato Astrain, Jesús and Durand Herrera, Daniel and Alaminos Mingorance, Miguel and Campos Muñoz, Antonio Jesús and Sánchez-Montesinos García, Indalecio and Campos Sánchez, Fernando},
}