@misc{10481/89466,
year = {2017},
month = {8},
url = {https://hdl.handle.net/10481/89466},
abstract = {Eggs obtained from the uteri of female nematodes, genetically identified as Contracaecum multipapillatum s.l., found in a brown pelican (Pelecanus occidentalis) from Bahía de La Paz, Gulf of California, Mexico, were used to study the early developmental stages of this anisakid by scanning electron microscopy (SEM). Egg dimensions were approximately 54 x 45 μm measured by SEM. Observation of the eggs revealed an outer surface of fibrous appearance. The newly hatched larvae were ensheathed and highly motile. Observation with SEM showed that the sheaths of the larvae were striated and revealed an excretory pore and a cleft near the anterior end of the sheath, presumably to facilitate the opening of the sheath for the emergence of the larva. The hatched larvae were placed in nutritive culture medium, where they grew within their sheath, some exsheathing completely two weeks later. The surface patterns of the sheath and the cuticle of the exsheathed larvae were clearly different. Although they did not moult during culture, SEM revealed a morphology typical of third-stage larvae of Contracaecum from fish, as previously observed by optical microscopy. Thus, we suggest that newly hatched larvae from eggs of C. multipapillatum are third larval stage but with sheath of the second larval stage, as occurs in other anisakids.},
organization = {This work has been funded by the Ministerio de Economía y Competitividad (Spain) and European Regional Development Fund (ERDF) (DMF, FJA and RB; grant number CGL2013-47725-P); and by the Instituto Politécnico Nacional, México (IVVand SHT; grant number SIP20141443).},
publisher = {Springer-Verlag GmbH Germany},
keywords = {Contracaecum multipapillatum},
keywords = {Egg},
keywords = {Larval early development},
keywords = {Aquatic life cycle},
keywords = {Morphology},
keywords = {Scanning electron microscopy},
title = {A scanning electron microscopy study of early development in vitro of Contracaecum multipapillatum s.l. (Nematoda: Anisakidae) from a brown pelican (Pelecanus occidentalis) from the Gulf of California, Mexico},
doi = {10.1007/s00436-017-5583-y},
author = {Molina Fernández, Dolores and Valles Vega, Isabel and Hernández Trujillo, Sergio and Adroher Auroux, Francisco Javier and Benítez Rodríguez, Rocío},
}