@misc{10481/88591,
year = {2019},
month = {8},
url = {https://hdl.handle.net/10481/88591},
abstract = {miRNAs play pivotal roles in various biological contexts, particularly in disease. Notably, miRNA-21 is implicated in severe pathological conditions, necessitating accurate quantification for medical diagnostics. Existing miRNA quantification techniques often fall short. Here, we introduce a novel, dynamic chemistry-based, PCR-free method for rapid and cost-effective miRNA-21 identification and quantification directly from tumor cells, employing FACS and fluorescent microplate assays. This approach, utilizing bead-based reagents, enables precise quantification of miR-21 molecules in MDA-MB-468 and H1975 tumor cells.},
organization = {This research was supported by the Regional Government, Spain(BIO-1778) and the Ministry of Economy and Competitiveness, Spain(BIO2016-80519-R – FJLD is supported by Torres Quevedo fellowship,SpainPTQ-16-08597).ADGandARRaresupportedbySpanishMinistryof Education, Spain (PhD scholarships FPU14/02181 and FPU15/06418, respectively). AMR thanks DestiNA Genomics, Spain and theUniversity of Granada, Spain for a PhD scholarship (P26 KnowledgeTransferProgramme)DMPacknowledgestheUniversityofGranadaforhis predoctoral research contract. SD's work was supported by in-tramural funding from the University of Trento, Italy. DestiNA's staffand SD thank the miRNADisEASY Consortium (EU, European UnionH2020-MSCA-RISE 2015 (Grant no. 690866)). We would also like tothank all the healthy volunteers who participated in this study.},
publisher = {Elsevier},
title = {PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells},
doi = {https://doi.org/10.1016/j.talanta.2019.03.039},
author = {Delgado-Gonzalez, Antonio and Robles Remacho, Agustín and Marín Romero, Antonio and De Miguel Pérez, Diego and Guardia Monteagudo, Juan José and Fara, Mario A. and Sánchez Martín, Rosario María and Díaz Mochón, Juan José},
}