@misc{10481/87518,
year = {2015},
month = {10},
url = {https://hdl.handle.net/10481/87518},
abstract = {The production of peptides exhibiting Angiotensin I-converting enzyme (ACE)-inhibitory activity from discarded Mediterranean fish species such as sardine, horse mackerel, axillary seabream, bogue and small-spotted catshark was studied. The evolution of the ACE-inhibitory activity with the degree of hydrolysis (DH) of protein hydrolysates was also investigated. Hydrolysates of horse mackerel and small-spotted catshark, both obtained with the simultaneous addition of subtilisin and trypsin, showed the highest antihypertensive activity (IC50 of 279 and 302μg/mL, respectively). For horse mackerel hydrolysate, fraction B (130-2350Da) exhibited the highest ACE-inhibitory activity (IC50=85μg/mL). In the case of small-spotted catshark hydrolysate, fraction D (<470Da) presented the lowest IC50 value (27μg/mL). In addition, 14 novel ACE-inhibitory peptides were identified in horse mackerel and small-spotted catshark hydrolysates. The peptide VAMPF, identified in fraction D of small-spotted catshark hydrolysate, is one of the most promising peptides according to its low IC50 value obtained by the QSAR-model (IC50=0.44μM)},
organization = {Spanish National Plan I + D + i (CTQ2011-23009)},
organization = {Andalusian Government (project P12-AGR-1993).},
publisher = {ELSEVIER},
keywords = {Fish discards},
keywords = {Enzymatic hydrolysis},
keywords = {SEC fractionation},
keywords = {ACE-inhibitory activity},
keywords = {Bioactive peptides},
title = {Production and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides from Mediterranean fish discards},
doi = {10.1016/j.jff.2015.06.062},
author = {García Moreno, Pedro Jesús and Espejo Carpio, Francisco Javier and Guadix Escobar, Antonio María and Guadix Escobar, Emilia María},
}