@misc{10481/87398,
year = {2009},
month = {8},
url = {https://hdl.handle.net/10481/87398},
abstract = {A 2-D-HPLC/CE method was developed to separate and characterize more in depth the
phenolic fraction of olive oil samples. The method involves the use of semi-preparative
HPLC (C18 column 250 10 mm, 5 mm) as a first dimension of separation to isolate
phenolic fractions from commercial extra-virgin olive oils and CE coupled to TOF-MS
(CE-TOF-MS) as a second dimension, to analyze the composition of the isolated fractions.
Using this method, a large number of compounds were tentatively identified, some
of them by first time, based on the information concerning high mass accuracy and the
isotopic pattern provided by TOF-MS analyzer together with the chemical knowledge and
the behavior of the compounds in HPLC and CE. From these results it can be concluded
that 2-D-HPLC-CE-MS provides enough resolving power to separate hundreds of
compounds from highly complex samples, such as olive oil. Furthermore, in this paper,
the isolated phenolic fractions have been used for two specific applications: quantification
of some components of extra-virgin olive oil samples in terms of pure fractions, and
in vitro studies of its anti-carcinogenic capacity.},
publisher = {Wiley},
title = {A 2-D-HPLC-CE platform coupled to ESITOF- MS to characterize the phenolic fraction in olive oil},
doi = {10.1002/elps.200800807},
author = {García Villalba, Rocío and Carrasco Pancorbo, Alegría and Vázquez-Martín, Alejandro and Oliveras-Ferraros, Cristina and Menéndez, Javier A. and Segura Carretero, Antonio and Fernández Gutiérrez, Alberto},
}