@misc{10481/70544,
year = {2021},
url = {http://hdl.handle.net/10481/70544},
abstract = {Skeletal muscles represent 40% of body mass and its native regenerative capacity can be
permanently lost after a traumatic injury, congenital diseases, or tumor ablation. The absence of
physiological regeneration can hinder muscle repair preventing normal muscle tissue functions.
To date, tissue engineering (TE) represents one promising option for treating muscle injuries and
wasting. In particular, hydrogels derived from the decellularized extracellular matrix (dECM) are
widely investigated in tissue engineering applications thanks to their essential role in guiding muscle
regeneration. In this work, the myogenic potential of dECM substrate, obtained from decellularized
bovine pericardium (Tissuegraft Srl), was evaluated in vitro using C2C12 murine muscle cells.
To assess myotubes formation, the width, length, and fusion indexes were measured during the
differentiation time course. Additionally, the ability of dECM to support myogenesis was assessed by
measuring the expression of specific myogenic markers: α-smooth muscle actin (α-sma), myogenin,
and myosin heavy chain (MHC). The results obtained suggest that the dECM niche was able to
support and enhance the myogenic potential of C2C12 cells in comparison of those grown on a
plastic standard surface. Thus, the use of extracellular matrix proteins, as biomaterial supports, could
represent a promising therapeutic strategy for skeletal muscle tissue engineering.},
organization = {Tissuegraft Srl},
organization = {University of Piemonte
Orientale, FAR 2017 “Development of innovative biological materials for the functional regeneration
of cardiac tissue models”},
publisher = {MDPI},
keywords = {Tissue engineering},
keywords = {Decellularized pericardium},
keywords = {Extracellular matrix},
keywords = {Skeletal muscle},
keywords = {Myogenic differentiation},
title = {Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium},
doi = {10.3390/ijms22179406},
author = {Carton, Flavia and Di Francesco, Dalila and Fusaro, Luca and Zanella, Emma and Apostolo, Claudio and Oltolina, Francesca and Cotella, Diego and Prat, María and Boccafoschi, Francesca},
}