@misc{10481/70508,
year = {2021},
month = {7},
url = {http://hdl.handle.net/10481/70508},
abstract = {A 98.1 Kb genomic region from B. pumilus 15.1, a strain isolated as an entomopathogen
toward C. capitata, the Mediterranean fruit fly, has been characterised in search of potential virulence
factors. The 98.1 Kb region shows a high number of phage-related protein-coding ORFs. Two regions
with different phylogenetic origins, one with 28.7 Kb in size, highly conserved in Bacillus strains,
and one with 60.2 Kb in size, scarcely found in Bacillus genomes are differentiated. The content of
each region is thoroughly characterised using comparative studies. This study demonstrates that
these two regions are responsible for the production, after mitomycin induction, of a phage-like
particle that packages DNA from the host bacterium and a novel phage for B. pumilus, respectively.
Both the phage-like particles and the novel phage are observed and characterised by TEM, and
some of their structural proteins are identified by protein fingerprinting. In addition, it is found that
the phage-like particle shows bacteriocin activity toward other B. pumilus strains. The effect of the
phage-like particles and the phage in the toxicity of the strain toward C. capitata is also evaluated.},
organization = {Andalucian Operative Program	B-BIO-081-UGR18},
publisher = {MDPI},
keywords = {Bacillus pumilus},
keywords = {Bacteriophage},
keywords = {Bacteriocin activity},
keywords = {PBSX},
keywords = {Entomopathogen},
title = {Bacillus pumilus 15.1, a Strain Active against Ceratitis capitata, Contains a Novel Phage and a Phage-Related Particle with Bacteriocin Activity},
doi = {10.3390/ijms22158164},
author = {Fernández Fernández, Alberto and Osuna Carrillo De Albornoz, Antonio and Vílchez Tornero, Susana},
}