@misc{10481/67255,
year = {2019},
month = {5},
url = {http://hdl.handle.net/10481/67255},
abstract = {The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups ofpolymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were appliedafter dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-FlowReactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation butstored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanicalassessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysiswere performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51%for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteriareduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, thehighest complex modulus values at the resin-dentin interface over time. Regarding the mineralcontent, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associatedto the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPsapplication permitted to observe remineralization of the etched and non-resin infiltratedcollagen layer, and bacteria were scarcely encountered. The combined antibacterial andremineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exertedan antibacterial role but did not remineralize the bonded interface. Undoped-NPs did notimprove the properties of the interfaces. Application of Zn-doped NPs during the bondingprocedure is encouraged.},
keywords = {dentin},
keywords = {degradation,},
keywords = {interface},
keywords = {nanoparticle},
keywords = {biofilm},
title = {Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation},
doi = {https://doi.org/10.1016/j.actbio.2020.05.002},
author = {Toledano Osorio, Manuel and Osorio Ruiz, Raquel and Sánchez Aguilera, Fátima and Medina Castillo, Antonio Luis and Toledano Pérez, Manuel and Osorio Ruiz, María Estrella and Acosta, Sergio and Chen, Ruoqiong and Aparicio, Conrado},
}