@misc{10481/63129,
year = {2019},
month = {10},
url = {http://hdl.handle.net/10481/63129},
abstract = {Background: Periodontitis, the most prevalent chronic inflammatory disease, has been related to cardiovascular
diseases. Autophagy provides a mechanism for the turnover of cellular organelles and proteins through a
lysosome-dependent degradation pathway. The aim of this research was to study the role of autophagy in
peripheral blood mononuclear cells from patients with periodontitis and gingival fibroblasts treated with a
lipopolysaccharide of Porphyromonas gingivalis. Autophagy-dependent mechanisms have been proposed in the
pathogenesis of inflammatory disorders and in other diseases related to periodontitis, such as cardiovascular
disease and diabetes. Thus it is important to study the role of autophagy in the pathophysiology of periodontitis.
Methods: Peripheral blood mononuclear cells from patients with periodontitis (n = 38) and without periodontitis (n =
20) were used to study autophagy. To investigate the mechanism of autophagy, we evaluated the influence of a
lipopolysaccharide from P. gingivalis in human gingival fibroblasts, and autophagy was monitored morphologically and
biochemically. Autophagosomes were observed by immunofluorescence and electron microscopy.
Results: We found increased levels of autophagy gene expression and high levels of mitochondrial reactive
oxygen species production in peripheral blood mononuclear cells from patients with periodontitis compared with
controls. A significantly positive correlation between both was observed. In human gingival fibroblasts treated with
lipopolysaccharide from P. gingivalis, there was an increase of protein and transcript of autophagy-related protein
12 (ATG12) and microtubule-associated protein 1 light chain 3 alpha LC3. A reduction of mitochondrial reactive
oxygen species induced a decrease in autophagy whereas inhibition of autophagy in infected cells increased
apoptosis, showing the protective role of autophagy.
Conclusion: Results from the present study suggest that autophagy is an important and shared mechanism in
other conditions related to inflammation or alterations of the immune system, such as periodontitis},
publisher = {Springer Nature},
keywords = {Reactive Oxygen Species Production},
keywords = {Periodontitis},
keywords = {Mitochondrial Reactive Oxygen Species},
keywords = {Gingival Fibroblast},
keywords = {Human Gingival Fibroblast},
title = {Autophagy in periodontitis patients and gingival fibroblasts: unraveling the link between chronic diseases and inflammation},
doi = {10.1186/1741-7015-10-122},
author = {Bullón, Pedro and Cordero, Mario and Quiles Morales, José Luis and Ramírez Tortosa, María Carmen and González-Alonso, Adrián and Alfonsi, Simona and García-Marín, Rocío and de Miguel, Manuel and Battino, Maurizio},
}