@misc{10481/61954,
year = {2019},
month = {5},
url = {http://hdl.handle.net/10481/61954},
abstract = {Background

Breast cancer ranks first in women, and is the second cause of death in this gender. In addition to genetics, the environment contributes to the development of the disease, although the factors involved are not well known. Among the latter is the influence of microorganisms and, therefore, attention is recently being paid to the mammary microbiota. We hypothesize that the risk of breast cancer could be associated with the composition and functionality of the mammary/gut microbiota, and that exposure to environmental contaminants (endocrine disruptors, EDCs) might contribute to alter these microbiota.
Methods

We describe a case-control clinical study that will be performed in women between 25 and 70 years of age. Cases will be women diagnosed and surgically intervened of breast cancer (stages I and II). Women with antecedents of cancer or advanced tumor stage (metastasis), or who have received antibiotic treatment within a period of 3 months prior to recruitment, or any neoadjuvant therapy, will be excluded. Controls will be women surgically intervened of breast augmentation or reduction. Women with oncological, gynecological or endocrine history, and those who have received antibiotic treatment within a period of 3 months prior to recruitment will also be excluded. Blood, urine, breast tissue and stool samples will be collected. Data regarding anthropometric, sociodemographic, reproductive history, tumor features and dietary habits will be gathered.

Metabolomic studies will be carried out in stool and breast tissue samples. Metagenomic studies will also be performed in stool and breast tissue samples to ascertain the viral, fungal, bacterial and archaea populations of the microbiota. Quantitation of estrogens, estrogen metabolites and EDCs in samples of serum, urine and breast tissue will also be performed.
Discussion: This is the first time that the contribution of bacteria, archaea, viruses and fungi together with their
alteration by environmental contaminants to the risk of breast cancer will be evaluated in the same study. Results
obtained could contribute to elucidate risk factors, improve the prognosis, as well as to propose novel intervention
studies in this disease.},
organization = {This work is funded by grants PI-0538-2017 (Junta de Andalucía, Spain, to LF) and Biomedical Research Networking Center-CIBER de Epidemiología y Salud Pública (CIBERESP) of the Institute of Health Carlos III -supported by European Regional Development Fund/FEDER (FIS-PI16/01812) (to MFF).},
publisher = {Springer Nature},
keywords = {Archaea},
keywords = {Bacteria},
keywords = {Breast cancer},
keywords = {Breast microbiota},
keywords = {Endocrine disruptors},
keywords = {Environmental pollutants},
keywords = {Fungi},
keywords = {Gut microbiota},
keywords = {Virus},
title = {Association of breast and gut microbiota dysbiosis and the risk of breast cancer: a case-control clinical study},
doi = {10.1186/s12885-019-5660-y},
author = {Plaza Díaz, Julio and Álvarez Mercado, Ana Isabel and Ruiz Marín, Carmen and Reina Pérez, Iris and Pérez Alonso, Alejandro José and Torné Poyatos, Pablo and Gallart Aragón, Tania and Sánchez-Barrón, María Teresa and Reyes Lartategui, S. de and García, Federico and Chueca-Porcuna, Natalia and Sáez Lara, María José and Robles-Sánchez, Candido and Fernández, Mariana F. and Fontana Gallego, Luis},
}