@misc{10481/61837,
year = {2019},
month = {7},
url = {http://hdl.handle.net/10481/61837},
abstract = {The present study was designed to develop a thermoreversible gel of Pluronic (P407)
loaded amphotericin B (AmB-gel) for the dermal and vaginal treatment of candidiasis. P407 was
used as a copolymer to exploit potential advantages related to increasing drug concentration in
the tissue layer in order to provide a local effect. Parameters including internal structure, swelling,
porosity, and short-term stability were determined. In addition, drug release profile and ex vivo
skin and vaginal permeation studies were carried out. Antifungal efficacy was evaluated against
strains of Candida spp. and atomic force microscopy (AFM) supported the results. The tolerance of
AmB-gel was studied by evaluating biomechanical properties of skin and determining the irritation
level in scarified rabbit skin supported by histological analysis. Results confirmed the development
of a thermoreversible AmB-gel with high porosity exhibiting Newtonian behavior at 4 ºC and
pseudoplasticity at 32 ºC as well as optimal stability for at least 90 days. The Amb-gel provided
a sustained drug release following a Boltzmann sigmoidal model. Non permeation was observed
in skin and vaginal mucosa, showing a high retained amount of AmB of 960.0 and 737.3 ug/g/cm2,
respectively. In vitro antifungal efficacy showed that AmB-gel was more effective than Free-AmB
in inhibiting strains of Candida spp. and these results were corroborated by AFM. Finally, tolerance
studies showed that its application did not induce skin irritation nor alter its biophysical properties.
Together, these results confirmed that AmB-gel could be proposed as a promising candidate for the
clinical status in the treatment of skin and vaginal candidiasis.},
organization = {This research was funded by L’Agència de Gestiód’Ajuts Universitarisi de Recerca (AGAUR)
grant number [SGR-2017 1744]. Lilian Sosa also expresses gratitude for the grant awarded by the Institute
of Nanoscience and Nanotechnology IN2UB number [2017.3.IN2UB.2] in the completion of her doctoral
thesis. Marcelle Silva-Abreu acknowledges the support from the Coordination for the Improvement Personnel
(CAPES-Brazil).},
publisher = {MDPI},
keywords = {Thermoreversible gel},
keywords = {Poloxamer 407},
keywords = {Amphotericin B},
keywords = {Skin and vaginal mucosa},
title = {Thermoreversible Gel-Loaded Amphotericin B for the Treatment of Dermal and Vaginal Candidiasis},
doi = {10.3390/pharmaceutics11070312},
author = {Jara Sosa, Lilian Elenea and Clares Naveros, Beatriz},
}