@misc{10481/61528,
year = {2019},
month = {3},
url = {http://hdl.handle.net/10481/61528},
abstract = {Fluorophores of the acridone family have been widely employed in many applications,
such as DNA sequencing, the detection of biomolecules, and themonitoring of enzymatic
systems, as well as being the bases of intracellular sensors and even antitumoral
agents. They have been widely used in fluorescence imaging due to their excellent
photophysical properties, in terms of quantum yield and stability. However, frequently, the
fluorescence emission data from acridones are not easily interpretable due to complex
excited-state dynamics. The formation of p-stacking aggregates and excimers and
excited-state proton transfer (ESPT) reactions usually result in emission features that
are dependent on the experimental conditions. Therefore, an in-depth understanding
of the dynamics involved in the excited-state transients of these dyes is mandatory for
their appropriate application. Herein, we synthesized and fully characterized different
2-methoxy-9-acridone dyes. Their transient fluorescence emission spectra exhibited a
complex dynamic behavior that can be linked to several excited-state reactions. We
performed a thorough study of the excited-state dynamics of these dyes by means of
time-resolved fluorimetry supported by computational calculations. All this allowed us to
establish a multistate kinetic scheme, involving an ESPT reaction coupled to an excimer
formation process. We have unraveled the rich dynamics behind this complex behavior,
which provides a better understanding of the excited states of these dyes.},
organization = {This work has been funded with Grant CTQ2017-
85658-R (Spanish Ministry of Economy and
Competitiveness; Agencia Estatal de Investigacion, AEI;
and European Regional Development Fund, ERDF) and
P12-FQM-790 (Junta de Andalucia).},
publisher = {Frontiers Media},
keywords = {Excited-state dynamics},
keywords = {Excited-state proton transfer},
keywords = {Computational photophysics},
keywords = {Fluorophores},
keywords = {Excimers},
title = {Coupled Excited-State Dynamics in N-Substituted 2-Methoxy-9-Acridones},
doi = {10.3389/fchem.2019.00129},
author = {González García, María del Carmen and Herrero-Foncubierta, Pilar and Castro, Silvia and Resa, Sandra and Álvarez Pez, José María and Miguel, Delia and Cuerva Carvajal, Juan Manuel and García-Fernández, Emilio and Orte Gutiérrez, Ángel},
}