@misc{10481/55399,
year = {2018},
month = {11},
url = {http://hdl.handle.net/10481/55399},
abstract = {The endometrium undergoes extensive changes to prepare for embryo implantation
and microRNAs (miRNAs) have been described as playing a significant role in the regulation
of endometrial receptivity. However, there is no consensus about the miRNAs involved in
mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early
secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients
with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the
mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early
to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles.
In total, 116 endometrial and 114 matched blood samples collected from two different population
cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were
identified in the mid-secretory vs. early secretory endometrium, while no differences were found in
the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and
infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among
discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory
endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family
members in mid-secretory endometrial functions.},
organization = {This research was funded by Estonian Ministry of Education and Research (grant IUT34-16); Enterprise
Estonia (grant EU48695); the EU-FP7 Eurostars program (grant NOTED, EU41564); the EU-FP7 Marie Curie
Industry-Academia Partnerships and Pathways (IAPP, grant SARM, EU324509); Horizon 2020 innovation
programme (WIDENLIFE, 692065); grant from the University of Granada (Incorporación de jóvenes doctores) and
grant from the Spanish Ministry of Economy, Industry and Competitiveness–MINECO–(RYC-2016-21199 and
grant ENDORE SAF2017-87526).},
publisher = {MDPI},
keywords = {Endometrial receptivity},
keywords = {Infertility},
keywords = {MicroRNA},
keywords = {Recurrent implantation failure},
keywords = {Small RNA-seq},
title = {A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women},
author = {Rekker, Kadri and Altmae, Signe and Suhorutshenko, Marina and Peters, Maire and Martinez-Blanch, Juan F. and Codoñer, Francisco M. and Vilella, Felipe and Simón, Carlos and Salumets, Andres and Velthut-Meikas, Agne},
}