@misc{10481/55343,
year = {2018},
month = {9},
url = {http://hdl.handle.net/10481/55343},
abstract = {Previous work from our group has shown that Cd38-/- mice develop a milder
pristane-induced lupus disease than WT or Art2-/- counterparts, demonstrating a new role for
CD38 in promoting aberrant inflammation and lupus-like autoimmunity via a Transient Receptor
Potential Melastatin 2 (TRPM2)-dependent apoptosis-driven mechanism. In this study we asked
whether CD38 may play a role in the expression and function of regulatory B cells (IL-10-producing
B cells or B10 cells). In pristane-treated mice the frequency of spleen CD19+CD1dhiCD5+ B cells,
which are highly enriched in B10 cells, was significantly increased in Cd38-/- splenocytes compared
to WT, while the frequency of peritoneal plasmacytoid dendritic cells (pDCs), which are major type I
Interferon (IFN) producers, was greatly diminished. The low proportion of pDCs correlated with
lower amounts of IFN-α in the peritoneal lavage fluids of the Cd38-/- mice than of WT and Art2-/-
mice. Functional ex vivo assays showed increased frequencies of IL-10-producing B cells in Cd38-/-
splenocytes than in WT upon stimulation with an agonist anti-CD40 mAb. Overall these results
strongly suggest that Cd38-/- mice are better suited than WT mice to generate and expand regulatory
B10 cells following the appropriate stimulation.},
organization = {Work performed in the Sancho and Zubiaur labs was supported in part by the European Commission
in collaboration with the following Funding Agencies: (i) Junta de Andalucía, Consejería Innovación Ciencia y
Empresa y Consejería Educación y Ciencia, Project: PC08-CTS-04046 to Jaime Sancho and Mercedes Zubiaur,
and (ii) Ministerio de Economía y Competitividad (MINECO), Projects: SAF-2011-27261 and SAF-2017-89801-R to
Jaime Sancho and Mercedes Zubiaur. The stay of B.B. and G.R. in Sancho’s lab was supported by National Science
Foundation: Grant #HRD-0963629 (G-STEM). USA.; and U.S. Department of Education; Student Aid and Fiscal
Responsibility Act; Title III Grant (SAFRA, Part F). Grant SAF-2017-89801-R covers in part the costs to publish in
open access.},
publisher = {MDPI},
keywords = {Bregs},
keywords = {CD38},
keywords = {CD1dhi},
keywords = {lupus},
keywords = {IL-10},
keywords = {Autoimmunity},
keywords = {Inflammation},
title = {The Role of CD38 on the Function of Regulatory B Cells in a Murine Model of Lupus},
author = {Burlock, Brianna and Richardson, Gabrielle and García Rodríguez, Sonia and Olivares Guerrero, Salvador and Zubiaur, Mercedes and Sancho, Jaime},
}