@misc{10481/53177,
year = {2017},
month = {12},
url = {http://hdl.handle.net/10481/53177},
abstract = {Objective: Characterization of novel calcium and zinc loaded electrospun matrices for
periodontal regeneration. Materials and Methods: A polymethylmetacrylate-based
membrane was calcium or zinc loaded. Matrices were characterized morphologically by
atomic force and scanning electron microscopy, and mechanically probed by a
nanoindenter. Biomimetic calcium phosphate precipitation on polymeric tissues was
assessed. Cell viability tests were performed using oral mucosa fibroblasts. Data were
analyzed by Kruskal-Wallis and Mann-Whitney tests or by ANOVA and Student-
Newman-Keuls multiple comparisons. Results: Zinc and calcium loading on matrices
did not modify their morphology but increased nanomechanical properties and
decreased nanoroughness. Precipitation of calcium and phosphate on the matrices
surfaces was observed in zinc-loaded specimens. Matrices were found to be non-toxic to
cells in all the assays. Calcium and zinc-loaded scaffolds presented a very low cytotoxic
effect. Conclusions: Zinc-loaded membranes permit cells viability and promoted
mineral precipitation in physiological conditions. Based on the tested nanomechanical
properties and scaffold architecture, the proposed membranes may be suitable for cell
proliferation. Clinical Relevance: The ability of zinc-loaded matrices to promote
precipitation of calcium phosphate deposits, together with their observed non-toxicity
and its surface chemistry allowing covalent binding of proteins, may offer new
strategies for periodontal regeneration.},
organization = {Project MAT2014-52036-P supported by the Ministry of Economy and
Competitiveness (MINECO) and European Regional Development Fund (FEDER).},
keywords = {Regeneration},
keywords = {calcium},
keywords = {Zinc},
keywords = {nanopolymers},
keywords = {scaffolds},
title = {Novel potential scaffold for periodontal tissue engineering.},
doi = {10.1007/s00784-017-2072-8.},
author = {Osorio Ruiz, Raquel and Alfonso-Rodríguez, Camilo-Alfonso and Osorio Ruiz, María Estrella and Medina Castillo, Antonio Luis and Alaminos Mingorance, Miguel and Toledano Osorio, Manuel and Toledano Pérez, Manuel},
}