@misc{10481/39009,
year = {2015},
url = {http://hdl.handle.net/10481/39009},
abstract = {Introduction. The purpose of this study was to investigate the micro-morphology of the
resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin
adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli.
Materials and Methods. Extracted human third molars were sectioned to obtain dentin
surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT)
and Experimental (EXP) both from VOCO, were applied following the manufacturer's
instructions or after 37% phosphoric acid application. Resin-dentin interfaces were
analyzed with dye assisted confocal microscopy evaluation (CLSM), including the
calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal
microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently
resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex
and adhesive layer completely affected by fluorescein penetration (nanoleakage) through
the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling
promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the
hybrid complex porosity, and an increment of dentin mineralization. Load cycled
specimens treated with the xylenol orange technique produced a clearly outlined
fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied.},
organization = {This work was supported by grants MINECO/FEDER MAT2011-24551, MAT2014-52036-P, and CEI-Biotic UGR.},
publisher = {Microscopy Society of America},
keywords = {Dentin},
keywords = {Fluoresceine},
keywords = {Rodhamine},
keywords = {Xylenol orange},
keywords = {Confocal microscopy},
title = {Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling},
doi = {10.1017/S1431927615015317},
author = {Toledano Pérez, Manuel and Cabello Malagón, Inmaculada and Sánchez Aguilera, Fátima and Osorio Ruiz, María Estrella and Toledano Osorio, Manuel and Osorio Ruiz, Raquel},
}