@misc{10481/30691,
year = {2014},
url = {http://hdl.handle.net/10481/30691},
abstract = {Pseudomonas aeruginosa
is ubiquitously present in the environment and acts as an opportunistic pathogen on humans,
animals and plants. We report here the effects of the prebiotic polysaccharide inulin and its hydrolysed form FOS on this
bacterium. FOS was found to inhibit bacterial growth of strain PAO1, while inulin did not affect growth rate or yield in a
significant manner. Inulin stimulated biofilm formation, whereas a dramatic reduction of the biofilm formation was
observed in the presence of FOS. Similar opposing effects were observed for bacterial motility, where FOS inhibited the
swarming and twitching behaviour whereas inulin caused its stimulation. In co-cultures with eukaryotic cells (macrophages)
FOS and, to a lesser extent, inulin reduced the secretion of the inflammatory cytokines IL-6, IL-10 and TNF-
a
. Western blot
experiments indicated that the effects mediated by FOS in macrophages are associated with a decreased activation of the
NF-
k
B pathway. Since FOS and inulin stimulate pathway activation in the absence of bacteria, the FOS mediated effect is
likely to be of indirect nature, such as via a reduction of bacterial virulence. Further, this modulatory effect is observed also
with the highly virulent ptxS mutated strain. Co-culture experiments of P. aeruginosa with IEC18 eukaryotic cells showed
that FOS reduces the concentration of the major virulence factor, exotoxin A, suggesting that this is a possible mechanism
for the reduction of pathogenicity. The potential of these compounds as components of antibacterial and anti-inflammatory
cocktails is discussed.},
organization = {The authors acknowledge financial support from FEDER funds and Fondo Social Europeo through grants from the Spanish Ministry of Economy and Competitiveness (grants SAF2011-22922, SAF2011-22812) the Andalusian regional government Junta de Andalucía (grant CVI-7335) and the Centre of Networked
Biomedical Research on Hepatic and Digestive Diseases (CIBERehd) which is funded by the Carlos III Health Institute and the Ramón Areces Foundation, Spain.},
publisher = {Public Library of Science (PLOS)},
keywords = {Toll-like receptors},
keywords = {Biofilm formation},
keywords = {Twitcing motility},
keywords = {Epithelial-cells},
keywords = {Secretion system},
keywords = {Lung infection},
keywords = {Antimicrobial activity},
keywords = {Protein secretion},
keywords = {Gene-expression},
title = {Fructooligosacharides Reduce Pseudomonas aeruginosa PAO1 Pathogenicity through Distinct Mechanisms},
author = {Ortega-González, Mercedes and Sánchez De Medina López-Huertas, Fermín and Molina Santiago, Carlos Alberto and López-Posadas, Rocío and Pacheco, Daniel and Krell, Tino and Martínez-Augustín, Olga and Daddaoua, Abdelali},
}