TY  - GEN 
AU  - Cura, Carolina I.
AU  - Duffy, Tomas
AU  - Lucero, Raúl H.
AU  - Bisio, Margarita
AU  - Péneau, Julie
AU  - Jiménez-Coello, Matilde
AU  - Calabuig, Eva
AU  - Giménez, María J.
AU  - Valencia Ayala, Edward
AU  - Kjos, Sonia A.
AU  - Santalla, José
AU  - Mahaney, Susan M.
AU  - Cayo, Nelly M.
AU  - Nagel, Claudia
AU  - Barcán, Laura
AU  - Málaga Machaca, Edith S.
AU  - Acosta Viana, Karla Y.
AU  - Brutus, Laurent
AU  - Ocampo, Susana B.
AU  - Aznar, Christine
AU  - Cuba Cuba, César A.
AU  - Gürtler, Ricardo E.
AU  - Ramsey, Janine M.
AU  - Ribeiro, Isabela
AU  - VandeBerg, John L.
AU  - Yadon, Zaida E.
AU  - Osuna Carrillo De Albornoz, Antonio
AU  - Schijman, Alejandro G.
PY  - 2015
SN  - 1935-2735
UR  - http://hdl.handle.net/10481/36691
AB  - Background:

Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI–TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes...
AB  - Methods/Principal Findings:

The MTq-PCR has been evaluated in 39 cultured stocks and 307 biological samples from vectors, reservoirs and patients from different geographical regions and transmission cycles in comparison with a multi-locus...
AB  - Conclusions/Significance:

Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.
LA  - eng
PB  - Public Library of Science (PLOS)
KW  - Trypanosoma cruzi
KW  - Polymerase chain reaction
KW  - Genotyping
KW  - Chagas disease
KW  - Leishmania
KW  - Vector-borne diseases
KW  - Sequence databases
KW  - Parasitic diseases
TI  - Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples
DO  - 10.1371/journal.pntd.0003765
ER  -