TY  - GEN 
AU  - Rodríguez Ruiz, Teresa
AU  - Méndez Vales, Rosa
AU  - Campo, Ana del
AU  - Jiménez Gámiz, Pilar
AU  - Aptsiauri, Natalia
AU  - Garrido Torres-Puchol, Federico
AU  - Ruiz-Cabello, Francisco
PY  - 2007
SN  - 1471-2407
UR  - http://hdl.handle.net/10481/29167
AB  - Background
The inability of cancer cells to present antigen on the cell surface via MHC class I molecules is one of the mechanisms by which tumor cells evade anti-tumor immunity. Alterations of Jak-STAT components of interferon (IFN)-mediated...
AB  - Methods
Basal and IFN-induced expression of HLA class I antigens was analyzed by means of indirect immunofluorescence flow cytometry, Western Blot, RT-PCR, and quantitative real-time RT-PCR (TaqMan® Gene Expression Assays). In demethylation studies...
AB  - Results
Altered IFN-γ mediated HLA-class I induction was observed in two melanoma cells lines (ESTDAB-004 and ESTDAB-159) out of 57 studied, while treatment of these two cell lines with IFN-α led to normal induction of HLA class I antigen expression....
AB  - Conclusion
We observed two distinct mechanisms of loss of IFN-γ inducibility of HLA class I antigens in two melanoma cell lines. Our findings suggest that loss of HLA class I induction in ESTDAB-004 cells results from a defect in the earliest steps...
LA  - eng
PB  - Biomed Central
KW  - Antineoplastic agents
KW  - Epigenesis
KW  - Genes
KW  - HLA antigents
KW  - Melanoma
KW  - Skin neoplasms
KW  - IRF1 protein
KW  - Interferon regulatory factor-1
TI  - Distinct mechanisms of loss of IFN-gamma mediated HLA class I inducibility in two melanoma cell lines
ER  -