Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs González-Pozas, Federico Montes, Rosa López Onieva, Lourdes Romero, Tamara Domingo Reinés, Joan Real Luna, Pedro José Ramos Mejía, Verónica Ayllon, Verónica Studies with different animal models have shown that the Notch ligand DLL4 has a key role in the development of the embryonic vasculature. Here we describe the generation and characterization of a human embryonic stem cell line and an induced pluripotent stem cell line that constitutively express short hairpin RNAs targeting DLL4 mRNA. These cells present reduced DLL4 expression at both protein and mRNA level, as well as a reduced induction of DLL4 target genes. They represent an ideal tool to study the role of DLL4 in human embryonic vascular and hematopoietic development. 2025-01-10T12:17:13Z 2025-01-10T12:17:13Z 2016-05 journal article González-Pozas F, Montes R, López-Onieva L, Romero T, Domingo-Reinés J, Real PJ, Ramos-Mejía V, Ayllón V. Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs. Stem Cell Res. 2016 May;16(3):735-9. doi: 10.1016/j.scr.2016.04.005. Epub 2016 Apr 16. PMID: 27346199. https://hdl.handle.net/10481/98874 10.1016/j.scr.2016.04.005 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional