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      <dc:title>miR-122 direct detection in human serum by time-gated fluorescence imaging</dc:title>
      <dc:creator>García Fernández, Emilio</dc:creator>
      <dc:creator>González García, María del Carmen</dc:creator>
      <dc:creator>Pernagallo, Salvatore</dc:creator>
      <dc:creator>Ruedas-Rama, Maria Jose</dc:creator>
      <dc:creator>Fara, Mario Antonio</dc:creator>
      <dc:creator>López Delgado, Francisco Javier</dc:creator>
      <dc:creator>W. Dear, James</dc:creator>
      <dc:creator>Ilyine, Hugh</dc:creator>
      <dc:creator>Ress, Cristina</dc:creator>
      <dc:creator>Díaz Mochón, Juan José</dc:creator>
      <dc:creator>Orte Gutiérrez, Ángel</dc:creator>
      <dc:description>A simple method for direct detection of microRNAs (miRs) in human&#xd;
serum without the use of polymerase amplification is presented,&#xd;
achieving low miR-122 concentrations and importantly, discerning&#xd;
effectively single-base sequence mutations. The method is based&#xd;
on the capture of target miRs with synthetic peptide nucleic acid&#xd;
oligomers, dynamic chemical labelling, separation with quaternary&#xd;
amine microplatforms and detection using time-gated fluorescence&#xd;
imaging.</dc:description>
      <dc:date>2024-12-12T12:39:56Z</dc:date>
      <dc:date>2024-12-12T12:39:56Z</dc:date>
      <dc:date>2019-11-27</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>García Fernández, E. et. al.  Chem. Commun., 2019, 55, 14958. [https://doi.org/10.1039/C9CC08069D]</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/97955</dc:identifier>
      <dc:identifier>10.1039/C9CC08069D</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 4.0 Internacional</dc:rights>
      <dc:publisher>Royal Society of Chemistry</dc:publisher>
   </ow:Publication>
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