A New Personalized Cooling Protocol to Activate Brown Adipose Tissue in Young Adults Martínez Téllez, Borja Manuel Sánchez Delgado, Guillermo García Rivero, Yolanda Alcántara Alcántara, Juan Manuel Martínez Ávila, Wendy D. Muñoz Hernández, María Victoria Olza, Josune R. Boon, Mariëtte C. N. Rensen, Patrick Llamas Elvira, José Manuel R. Ruiz, Jonatan cooling vest PET/CT scan glucose uptake Brown adipose tissue (BAT) activity is induced when humans are exposed to cold. Therefore, cold exposure prior to the 18F-FDG-PET/CT scan is used as a tool to quantify BAT. Several cooling protocols, including fixed and personalized ones are currently in use. The aim of the present study was to determine the effect of a new personalized cooling protocol where the shivering threshold was measured on a separate day, on BAT volume and activity in young adults. A total of 47 adults (n = 28 women) aged 22 ± 2 years participated in the study. We determined participants’ shivering threshold (visually and self-reported) using a water perfused cooling vest in an air-conditioned cold room. 48–72 h later, participants wore the cooling vest set at ∼4◦C above the shivering threshold for 60min prior to injection of 18F-FDG and ∼5◦C above the shivering threshold for ∼60min after injection, until PET/CT scan. We quantified BAT following BARCIST 1.0 recommendations. We identified 40 participants (85%, n = 25 women) as PET+ and 7 (n = 3 women) as PET–. The PET+ group presented significantly higher BAT volume and activity than PET– group (all P < 0.05). PET+ women had higher BAT mean activity than PET+ men (SUVmean: 5.0 ± 1.6 vs. 3.6 ± 0.9 g/ml respectively, P = 0.003), and there were no significant sex differences in BAT volume (P = 0.161). A total of 9 out of 47 participants did not shiver during the shivering threshold test. Our findings are similar to previous cold-stimulated human BAT studies; therefore, we conclude that our personalized cooling protocol is able to activate BAT in young adults. 2024-11-26T07:42:17Z 2024-11-26T07:42:17Z 2017-11-02 journal article Martínez Téllez, B. et. al. Front. Physiol. 8:863. [https://doi.org/10.3389/fphys.2017.00863] https://hdl.handle.net/10481/97349 10.3389/fphys.2017.00863 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Frontiers Media