Application of Liquid Chromatography-Ion Mobility Spectrometry-Mass Spectrometry-Based Metabolomics to Investigate the Basal Chemical Profile of Olive Cultivars Differing in Verticillium dahliae Resistance Serrano García, Irene Martakos, Ioannis C. Olmo García, Lucía León, Lorenzo De la Rosa, Raúl Gómez Caravaca, Ana María Belaj, Angjelina Serrano, Alicia Dasenaki, Marilena E. Thomaidis, Nikolaos S. Carrasco Pancorbo, Alegría Olea europaea L. LC-MS profiling TIMS Olive roots Olive stems Olive leaves pathogen resistance Verticillium Wilt of Olive The limited effectiveness of current strategies to control Verticillium wilt of olive (VWO) prompts the need for innovative approaches. This study explores the basal metabolome of 43 olive cultivars with varying resistance levels to Verticillium dahliae, offering alternative insights for olive crossbreeding programmes. The use of an innovative UHPLC-ESI-TimsTOF MS/MS platform enabled the annotation of more than 70 compounds across different olive organs (root, stem, and leaf) and the creation of a preliminary compilation of TIMSCCSN2 experimental data for more reliable metabolite annotation. Moreover, it allowed the documentation of numerous isomeric species in the studied olive organs by resolving hidden compounds. Multivariate statistical analyses revealed significant metabolome variability between highly resistant and susceptible cultivars, which was further investigated through supervised PLS-DA. Key markers indicative of VWO susceptibility were annotated and characteristic compositional patterns were established. Stem tissue exhibited the highest discriminative capability, while root and leaf tissues also showed significant predictive potential. 2024-11-25T12:37:36Z 2024-11-25T12:37:36Z 2024-11-22 journal article https://hdl.handle.net/10481/97341 https://doi.org/10.1021/acs.jafc.4c07155 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional