Transcriptomics of Diphyllatea (CRuMs) from South Pacific crater lakes confirm new cryptic clades Galindo, Luis Javier Mathur, Varsha Frost, Hadleigh Torruella, Guifré Richards, Thomas A. Irwin, Nicholas A. T. Biogeography Collodictyon Crater lakes The Diphyllatea (CRuMs) are heterotrophic protists currently divided into three distinct clades (Diphy I–III). Diphy I are biflagellates in the genus Diphylleia, whereas Diphy II and III represent cryptic clades comprising Collodictyon-type quadriflagellates that were recently distinguished based on rRNA gene phylogenies. Here, we isolated Diphyllatea from freshwater crater lakes on two South Pacific islands and generated high-quality transcriptomes from species representing each clade, including the first transcriptomic data from Diphy III. Phylogenomic analyses support the separation of Diphy II and III, while transcriptome completeness highlights the utility of these data for future studies. Lastly, we discuss the biogeography and ecology of Diphyllatea on these remote islands. 2024-10-29T09:27:57Z 2024-10-29T09:27:57Z 2024-09-28 journal article Galindo, L.J., Mathur, V., Frost, H., Torruella, G., Richards, T.A. & Irwin, N.A.T. (2024) Transcriptomics of Diphyllatea (CRuMs) from South Pacific crater lakes confirm new cryptic clades. Journal of Eukaryotic Microbiology, 00, e13060. Available from: https://doi.org/10.1111/jeu.13060 https://hdl.handle.net/10481/96435 10.1111/jeu.13060 eng info:eu-repo/grantAgreement/EC/H2020/MSC 101022101 http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Institute of Electrical and Electronics Engineers (IEEE)