Metabolic profiling and antioxidant activity of fenugreek seeds cultivars ‘Giza 2’ and ‘Giza 30’ compared to other geographically-related seeds Hassan Mekky, Reham Abdel Sattar, Essam Abdulla, Maha-Hamadien Segura Carretero, Antonio Al-Khayal, Khayal Eldehna, Wagdy M. Contreras, María del Mar Fenugreek Flavonoids Furostanol saponins This study addresses a comparative comprehensive metabolic profiling of two Egyptian cultivars of fenugreek (Trigonella foenum-graecum L.) seeds ‘Giza 2’ and ‘Giza 30’ via RP-HPLC-DAD-QTOF-MS and MS/MS. Briefly, 126 metabolites were detected in the samples under investigation, being classified into hydroxybenzoic acids (8), hydroxycinnamic acids (7), flavonoids (49 with a predominancy of flavones in particular apigenin derivatives), coumarins (1), furostanol saponins (17), alkaloids (2), amino acids (11), peptides (2), jasmonates (6), nucleosides (30), organic acids (16), terpenoids (1), and sugars (3). In addition, the total phenolic content and antioxidant activity were determined and compared with other geographically related seeds (chickpea Giza-1, sesame Giza- 32, and linseed, Giza-10), showing slight differences among them but higher values than the other geographically related seeds that were segregated from them upon chemometric analysis. This is the first comprehensive metabolic profiling of these cultivars, presenting an initial account of some metabolites found in Fabaceae, such as apigenin di C pentoside, with a significant occurrence of biologically active furostanol saponins. It gives a prospect of fenugreeks richness of bioactive metabolites as an essential functional food that could add value to the food and nutraceutical industries' sustainability. 2024-10-04T11:21:53Z 2024-10-04T11:21:53Z 2024-09-07 journal article R.H. Mekky et al. Food Chemistry: X 24 (2024) 101819 [https://doi.org/10.1016/j.fochx.2024.101819] https://hdl.handle.net/10481/95544 10.1016/j.fochx.2024.101819 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier