High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming inm. triceps brachii Casuso, Rafael A. Plaza Díaz, Julio Ruiz Ojeda, Francisco Javier Aragón Vela, Jerónimo Robles-Sánchez, Candido Nordsborg, Nikolai B Hebberecht, Marina Salmerón Febres, Luis Miguel Rodríguez Huertas, Jesús Francisco We aimed to test whether high-intensity high-volume training (HIHVT) swimming would induce more robust signaling than sprint interval training (SIT) swimming within the m. triceps brachii due to lower metabolic and oxidation. Nine well-trained swimmers performed the two training procedures on separate randomized days. Muscle biopsies from m. triceps brachii and blood samples were collected at three different time points: a) before the intervention (pre), b) immediately after the swimming procedures (post) and c) after 3 h of rest (3 h). Hydroperoxides, creatine kinase (CK), and lactate dehydrogenase (LDH) were quantified from blood samples, and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) and the AMPKpTHR172/AMPK ratio were quantified by Western blot analysis. PGC-1α, sirtuin 3 (SIRT3), superoxide-dismutase 2 (SOD2), and vascular endothelial growth factor (VEGF) mRNA levels were also quantified. SIT induced a higher release of LDH (p < 0.01 at all time points) and CK (p < 0.01 at post) than HIHVT, but neither SIT nor HIHVT altered systemic hydroperoxides. Additionally, neither SIRT3 nor SOD2 mRNA levels increased, while PGC-1α transcription increased at 3 h after SIT (p < 0.01) and after HIHVT (p < 0.001). However, PGC-1α protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h (p < 0.05). In addition, VEGF transcription was higher in response to HIHVT (p < 0.05). In conclusion, SIT induces higher muscular stress than HIHVT without increasing systemic oxidation. In addition, HIHVT may induce more robust oxidative adaptations through PGC-1α and AMPK. 2024-10-04T10:04:41Z 2024-10-04T10:04:41Z 2017-10-03 journal article Casuso RA, Plaza-Díaz J, Ruiz-Ojeda FJ, Aragón-Vela J, Robles-Sanchez C, Nordsborg NB, et al. (2017) High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii. PLoS ONE 12(10): e0185494. https://doi.org/10.1371/journal.pone.0185494 https://hdl.handle.net/10481/95530 10.1371/journal.pone.0185494 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Universidad Pontificia de Comillas