<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/95283">
      <dc:title>Induction and real-time ultrasonic monitoring of 3D cartilage-like tissue by a low shear stresses-based bioreactor</dc:title>
      <dc:creator>Martínez Moreno, Daniel</dc:creator>
      <dc:creator>Callejas Zafra, Antonio Manuel</dc:creator>
      <dc:creator>Jiménez González, Gema</dc:creator>
      <dc:creator>Gálvez Martín, Patricia</dc:creator>
      <dc:creator>Rus Carlborg, Guillermo</dc:creator>
      <dc:creator>Marchal Corrales, Juan Antonio</dc:creator>
      <dc:subject>Scaffolds</dc:subject>
      <dc:subject>Infrapatellar fat pad mesenchymal stem cells (IPFP-MSCs)</dc:subject>
      <dc:subject>1,4-Butanediol thermoplastic polyurethane (bTPUe)</dc:subject>
      <dc:description>Osteoarthritis is a significant socioeconomic illness that mainly affects the articular&#xd;
cartilage, a tissue with a low capacity for self-healing, making it an ideal target&#xd;
for regenerative medicine and tissue engineering. Current interventions to treat&#xd;
cartilage injuries may not be completely effective. In this study, we have developed&#xd;
a novel bioreactor that creates viscous shear stress by flow perfusion. This bioreactor&#xd;
could induce ex vivo maturation of biomimetic 3D cartilage scaffolds, providing a&#xd;
potential solution to this problem. Infrapatellar fat pad mesenchymal stem cells&#xd;
(IPFP-MSCs) were used as a cellular source of the functionalized 3D scaffolds made&#xd;
of 1,4-butanediol thermoplastic polyurethane (bTPUe) modified with pyrene&#xd;
butyric acid (PBA). Our results indicate that our bioreactor induced chondrogenic&#xd;
differentiation, as confirmed by DNA quantification, extracellular matrix&#xd;
determination, and metabolic assay, without any conditioned medium. To control&#xd;
the biomechanical stimulation on IPFP-MSCs, a low-intensity ultrasonic transmission&#xd;
system has been developed and embedded in the bioreactor. Combined with a finite&#xd;
element model (FEM), tissue growth and differentiation can be deconvoluted in realtime&#xd;
from the recorded ultrasonic propagation and interaction across the graft. The&#xd;
FEM reconstructs this complex interaction. This is the first time a low-shear stressbased&#xd;
bioreactor has been reported to not only induce chondrogenic evolution but&#xd;
also monitor it in real time.</dc:description>
      <dc:date>2024-10-01T06:33:02Z</dc:date>
      <dc:date>2024-10-01T06:33:02Z</dc:date>
      <dc:date>2024-07-12</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Martínez Moreno, D. et. al. Int J Bioprint. 2024;10(4):3389. [https://doi.org/10.36922/ijb.3389]</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/95283</dc:identifier>
      <dc:identifier>10.36922/ijb.3389</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 4.0 Internacional</dc:rights>
      <dc:publisher>AccScience Publishing</dc:publisher>
   </ow:Publication>
</rdf:RDF>