Multiomic-based immune response profiling in migraine, vestibular migraine and Meniere’s disease Cruz-Granados, Pablo Frejo, Lidia Pérez Carpena, Patricia Amor Dorado, Juan Carlos Dominguez Durán, Emilio Fernandez Nava, Maria Jose Batuecas Caletrio, Angel Haro Hernández, Elisheba Martínez Martínez, Marta López Escámez, José Antonio Autoinflammation Cytokines Meniere disease Migraine (MI) is the most common neurological disease, affecting with 20% of the world population. A subset of 25% of MI patients showcase concurrent vestibular symptoms, which may classify as vestibular migraine (VM). Meniere’s disease (MD) is a complex inner ear disorder defined by episodes of vertigo associated with tinnitus and sensorineural hearing loss with a significant autoimmune/ autoinflammatory contribution, which symptoms overlap with VM. Blood samples from 18 patients with MI (5), VM (5) and MD (8) and 6 controls were collected and compared in a case–control study. Droplet-isolated nuclei from mononuclear cells used to generate scRNAseq and scATACseq data sets from MI, VM and MD. MI and VM have no differences in their immune transcriptome; therefore, they were considered as a single cluster for further analyses. Natural Killer (NK) cells transcriptomic data support a polarisation triggered by Type 1 innate immune cells via the release of interleukin (IL)-12, IL-15 and IL-18. According to the monocyte scRNAseq data, there were two MD clusters, one inactive and one driven by monocytes. The unique pathways of the MI + VM cluster were cellular responses to metal ions, whereas MD monocyte-driven cluster pathways showed responses to biotic stimuli. MI and MD have different immune responses. These findings support that MI and VM have a Type 1 immune lymphoid cell response, and that there are two clusters of MD patients, one inactive and one Monocyte-driven. 2024-09-26T11:47:51Z 2024-09-26T11:47:51Z 2024-09-18 journal article Cruz Granados, P. et. al. Immunology. 2024;1–12. [https://doi.org/10.1111/imm.13863] https://hdl.handle.net/10481/95165 10.1111/imm.13863 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley Online Library