MicroRNA Dysregulation in Early Breast Cancer Diagnosis: A Systematic Review and Meta-Analysis Garrido Palacios, Alejandro Rojas Carvajal, Ana María Núñez Negrillo, Ana María Cortés Martín, Jonathan Sánchez García, Juan Carlos Aguilar Cordero, Josefa Micro-RNA Cancer Biomarkers Breast cancer continues to be the leading cause of death in women worldwide. Mammography, which is the current gold standard technique used to diagnose it, presents strong limitations in early ages where breast cancer is much more aggressive and fatal. MiRNAs present in numerous body fluids might represent a new line of research in breast cancer biomarkers, especially oncomiRNAs, known to play an important role in the suppression and development of neoplasms. The aim of this systematic review and meta-analysis was to evaluate dysregulated miRNA biomarkers and their diagnostic accuracy in breast cancer. Two independent researchers reviewed the included studies according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. A protocol for this review was registered in PROSPERO with the registration number “CRD42021256338”. Observational case-control-based studies analyzing concentrations of microRNAs which have been published within the last 10 years were selected, and the concentrations of miRNAs in women with breast cancer and healthy controls were analyzed. Random-effects meta-analyses of miR-155 were performed on the studies which provided enough data to calculate diagnostic odds ratios. We determined that 34 microRNAs were substantially dysregulated and could be considered biomarkers of breast cancer. Individually, miR-155 provided better diagnostic results than mammography on average. However, when several miRNAs are used to screen, forming a panel, sensitivity and specificity rates improve, and they can be associated with classic biomarkers such us CA-125 or CEA. Based on the results of our meta-analysis, miR-155 might be a promising diagnostic biomarker for this patient population. 2024-09-23T10:46:21Z 2024-09-23T10:46:21Z 2023-05-05 journal article Garrido-Palacios, A.; Rojas Carvajal, A.M.; Núñez-Negrillo, A.M.; Cortés-Martín, J.; Sánchez-García, J.C.; Aguilar-Cordero, M.J. MicroRNA Dysregulation in Early Breast Cancer Diagnosis: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci. 2023, 24, 8270. https://doi.org/10.3390/ijms24098270 https://hdl.handle.net/10481/94890 10.3390/ijms24098270 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI