Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis Agrawal, Smriti Anderson, Per Durbeej, Madeleine Van Rooijen, Nico Ivars, Fredrik Opdenakker, Ghislain Sorokin, Lydia M. Dystroglycan Parenchymal basement membrane Leukocyte extravasation Autoimmune encephalomyelitis German (grant nos. So285/5-1, So285/5-2) and Swedish Research Councils (grant nos. K2005-06X-14184-04A, 621-2001- 2142), Alfred Österlunds, Knut and Alice Wallenbergs (grant no. 2002.0056), the Greta and Johan Kocks Foundations, the Fund for Scientifi c Research-Flanders, the “Geconcerteerde OnderzoeksActies,” and the Charcot Foundation, Belgium. The endothelial cell monolayer of cerebral vessels and its basement membrane (BM) are ensheathed by the astrocyte endfeet, the leptomeningeal cells, and their associated parenchymal BM, all of which contribute to establishment of the blood–brain barrier (BBB). As a consequence of this unique structure, leukocyte penetration of cerebral vessels is a multistep event. In mouse experimental autoimmune encephalomyelitis (EAE), a widely used central nervous system infl ammatory model, leukocytes fi rst penetrate the endothelial cell monolayer and underlying BM using integrin 𝛃 1-mediated processes, but mechanisms used to penetrate the second barrier defi ned by the parenchymal BM and glia limitans remain uninvestigated. We show here that macrophage-derived gelatinase (matrix metalloproteinase [MMP]-2 and MMP-9) activity is crucial for leukocyte penetration of the parenchymal BM. Dystroglycan, a transmembrane receptor that anchors astrocyte endfeet to the parenchymal BM via high affi nity interactions with laminins 1 and 2, perlecan and agrin, is identifi ed as a specifi c substrate of MMP-2 and MMP-9. Ablation of both MMP-2 and MMP-9 in double knockout mice confers resistance to EAE by inhibiting dystroglycan cleavage and preventing leukocyte infi ltration. This is the fi rst description of selective in situ proteolytic damage of a BBB-specifi c molecule at sites of leukocyte infi ltration. 2024-09-23T09:10:27Z 2024-09-23T09:10:27Z 2006-04 journal article Agrawal, Smriti et al. “Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.” The Journal of experimental medicine vol. 203,4 (2006): 1007-19. doi:10.1084/jem.20051342 https://hdl.handle.net/10481/94870 10.1084/jem.20051342 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional The Rockefeller University Press