Characterization of Phenolic Compounds in Extra Virgin Olive Oil from Granada (Spain) and Evaluation of Its Neuroprotective Action López Bascón, María Asunción Moscoso Ruiz, Inmaculada Quirantes Piné, Rosa Del Pino-García, Raquel López Gámez, Gloria Justicia Rueda, Andrea Verardo, Vito Quiles Morales, José Luis Extra virgin olive oil Phenolic compounds Neuroprotective potential The olive oil sector is a fundamental food in the Mediterranean diet. It has been demonstrated that the consumption of extra virgin olive oil (EVOO) with a high content of phenolic compounds is beneficial in the prevention and/or treatment of many diseases. The main objective of this work was to study the relationship between the content of phenolic compounds and the in vitro neuroprotective and anti-inflammatory activity of EVOOs from two PDOs in the province of Granada. To this purpose, the amounts of phenolic compounds were determined by liquid chromatography coupled to mass spectrometry (HPLC–MS) and the inhibitory activity of acetylcholinesterase (AChE) and cyclooxygenase-2 (COX-2) enzymes by spectrophotometric and fluorimetric assays. The main families identified were phenolic alcohols, secoiridoids, lignans, flavonoids, and phenolic acids. The EVOO samples with the highest total concentration of compounds and the highest inhibitory activity belonged to the Picual and Manzanillo varieties. Statistical analysis showed a positive correlation between identified compounds and AChE and COX-2 inhibitory activity, except for lignans. These results confirm EVOO’s compounds possess neuroprotective potential. 2024-09-19T09:00:47Z 2024-09-19T09:00:47Z 2024-04-30 journal article López-Bascón, M.A.; Moscoso-Ruiz, I.; Quirantes-Piné, R.; del Pino-García, R.; López-Gámez, G.; Justicia-Rueda, A.; Verardo, V.; Quiles, J.L. Characterization of Phenolic Compounds in Extra Virgin Olive Oil from Granada (Spain) and Evaluation of Its Neuroprotective Action. Int. J. Mol. Sci. 2024, 25, 4878. https://doi.org/10.3390/ijms25094878 https://hdl.handle.net/10481/94713 10.3390/ijms25094878 eng info:eu-repo/grantAgreement/EC/NextGenerationEU/FJC2021-047564-I http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI