Biofortification of Cucumbers with Iron Using Bio-Chelates Derived from Spent Coffee Grounds: A Greenhouse Trial Cervera Mata, Ana Gloria Lara-Ramos, Leslie Rufián Henares, José Ángel Fernández Bayo, Jesús Dionisio Delgado Calvo-Flores, Gabriel Fernández Arteaga, Alejandro Bio-chelates Coffee by-products Hydrochar The transformation of spent coffee grounds (SCGs) into hydrochars has been extensively studied in recent years to explore their potential in biofortifying foods and mitigating the plant toxicity associated with SCGs. This study aimed to evaluate the effects of adding activated (ASCG and AH160) and functionalized SCGs, as well as SCG-derived hydrochars (ASCG-Fe and AH160-Fe), on cucumber production and plant iron content. To achieve this, SCGs and SCG-derived hydrochars activated and functionalized with Fe were incorporated into cucumber crops grown in a greenhouse over multiple harvests. Among the treatments, SCG-Fe proved to be the most promising for cucumber production, yielding an average of 25 kg of cumulative production per treatment across three harvests. Regarding iron content, the average results across all harvests showed that SCGs and functionalized SCG-hydrochars matched the performance of the commercial chelate (0.108 vs. 0.11 mg Fe/100 g fresh weight). However, in subsequent harvests, iron appeared to leach out, with the activated bio-products (ASCG and AH160) leaving the highest iron reserves in the soil. Additionally, the hydrochar activated at 160 ºC demonstrated the highest utilization efficiency. In conclusion, the incorporation of SCG residues and second-generation residues (hydrochars) shows promise as agents for biofortifying cucumbers. 2024-09-12T10:21:31Z 2024-09-12T10:21:31Z 2024-09-09 journal article Cervera-Mata, A.; Lara-Ramos, L.; Rufián-Henares, J.Á.; Fernández-Bayo, J.; Delgado, G.; Fernández-Arteaga, A. Biofortification of Cucumbers with Iron Using Bio-Chelates Derived from Spent Coffee Grounds: A Greenhouse Trial. Agronomy 2024, 14, 2063. https://doi.org/10.3390/agronomy14092063 https://hdl.handle.net/10481/94387 10.3390/agronomy14092063 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI