Efficacy of topical application of corticosteroids in the remineralization of dental pulp tissue. A systematic review of the literature Vallecillo Rivas, Marta Fernández-Romero, Enrique Pérez-Segura, Michelle Toledano, Raquel Amar-Zetouni, Anisa Toledano Pérez, Manuel Vallecillo Rivas, María Cristina Corticosteroids Mesenchymal stem cells Mineralization Odontogenic differentiation Dental pulp The present study was supported by Grant PID2020–114694RB-I00 funded by MCIN/AEI 10.13039/501100011033. Objectives: The aim of this systematic review was to demonstrate the efficacy of topical application of corticosteroids in remineralization of dental pulp tissues to preserve their vitality and function. Data, sources and study selection: An electronic search was performed using MEDLINE by PubMed, EMBASE, Web of Science (WOS), and Scopus databases. The inclusion criteria were in vitro studies that employed dental pulp tissue obtained from extracted healthy permanent human teeth and were subjected to topical administration of corticosteroids and evaluated tissue remineralization by performing any mineralization assay. A total of 11 studies were selected for inclusion. PRISMA guidelines were followed, and the methodological quality and risk of bias of the included studies were evaluated using the RoBDEMAT guidelines. Also, tables were designed for data extraction, including tissue mineralization and osteogenic differentiation as primary and secondary outcomes, respectively. Conclusions: Alizarin Red S (ARS) has been able to demonstrate a possible mineralizing power of corticosteroids, applied at an adequate dose. The up-regulation of Alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OSP), sialophosphoprotein (DSPP), runt-related transcription factor 2 (RUNX2), collagen type 1 alpha 1 (COL1α1) and dentin matrix protein 1 (DMP-1) induced the osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Clinical significance: Deep carious lesions treatment is still challenging in restorative dentistry. Some treatments have been focused on dental pulp tissue remineralization to maintain the function and vitality. After corticosteroids topical application, mineral deposition and osteogenic differentiation have been detected. 2024-09-11T07:51:36Z 2024-09-11T07:51:36Z 2024-08-30 journal article Vallecillo-Rivas M, Fernández-Romero E, Pérez-Segura M, et al. Efficacy of topical application of corticosteroids in the remineralization of dental pulp tissue. A systematic review of the literature. J Dent. Published online August 30, 2024. doi:10.1016/j.jdent.2024.105333 https://hdl.handle.net/10481/94315 10.1016/j.jdent.2024.105333 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier