Tunable polymer-peptide hybrids for dentin tissue repair Toledano, Manuel Fernández-Romero, Enrique Aguilera, Fátima S. Osorio, Estrella Rodríguez-Santana, José Alejandro Garrido, Macarena Solís, Pedro Antonio García-Godoy, Franklin Osorio, Raquel Dentin Masson's trichrome staining Nanoparticles Raman Remineralization Tideglusib. Acknowledgements The present study was supported by Grant PID2020–114694RB-I00 funded by MCIN/AEI 10.13039/501100011033. This research is part of E. F-R.’s Ph.D. research study. Objectives: This study targets to assess the remineralization capability of conditioned dentin infiltrated with polymeric nanoparticles (NPs) doped with tideglusib (TDg) (TDg-NPs). Methods: Dentin conditioned surfaces were infiltrated with NPs and TDg-NPs. Bonded interfaces were created, stored for 24 h and submitted to mechanical and thermal challenging. Resin-dentin interfaces were evaluated through nanohardness, Masson's trichrome staining microscopy, and Raman analysis. Results: Dentin surfaces treated with TDg-NPs and load cycled produced higher nanohardness than the rest of the groups at the hybrid layer. At the bottom of the hybrid layer, all samples treated with TDg-NPs showed higher nanohardness than the rest of the groups. Active remineralization underneath the hybrid layer was detected in all groups after TDg application and load cycling, inducting new dentinal tubuli formation. After thermocycling, remineralization at the hybrid layer was not evidenced in the absence of NPs. Raman analysis showed increase mineralization, enriched carbonate apatite formation, and improved crosslinking and scaffolding of the collagen. Conclusions: Mechanical loading on the specimens obtained after TDg-NPs dentin infiltration inducts an increase of mineralization at the resin/dentin interface, indicating remineralization of peritubular and intertubular dentin with augmented crystallographic maturity in crystals. Enriched collagen quality was produced, generating an adequate matrix organization to promote apatite nucleation, after tideglusib infiltration. Clinical significance: At the present research, it has been proved the creation of reparative dentin, at the resin-dentin interface, after tideglusib dentin infiltration. Chemical stability, to favor integrity of the resin-dentin interface, is warranted in the presence of the TDg-NPs in the demineralized dentin collagen. 2024-09-02T11:34:06Z 2024-09-02T11:34:06Z 2024 journal article Toledano M, Fernández-Romero E, Aguilera FS, Osorio E, Rodríguez-Santana JA, Garrido M, Solís PA, García-Godoy F, Osorio R. Tunable polymer-peptide hybrids for dentin tissue repair. J Dent. 2024 Sep;148:105027. https://hdl.handle.net/10481/93789 10.1016/j.jdent.2024.105027 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier Ltd