Single-Molecule Identification of the Isomers of a Lipidic Antibody Activator Mallada, Benjamin Villalobos Romero, Federico Donoso, Beatriz Casares López, Raquel Longhi, Giovanna Mendieta-Moreno, Jesús I. Jiménez Martín, Alejandro Haidour, Ali Seepersaud, Ravin Rajagopal, Lakshmi de la Torre, Bruno Millán Delgado, Alba Cuerva, Juan M. Molecular structural elucidation can be accomplished by different techniques, such as nuclear magnetic resonance or X-ray diffraction. However, the former does not give information about the three-dimensional atomic arrangement, and the latter needs crystallizable solid samples. An alternative is direct, real-space visualization of the molecules by cryogenic scanning tunneling microscopy (STM). This technique is usually limited to thermally robust molecules because an annealing step is required for sample deposition. A landmark development has been the coupling of STM with electrospray deposition (ESD), which smooths the process and widens the scope of the visualization technique. In this work, we present the on-surface characterization of air-, light-, and temperature-sensitive rhamnopolyene with relevance in molecular biology. Supported by theoretical calculations, we characterize two isomers of this flexible molecule, confirming the potential of the technique to inspect labile, non-crystallizable compounds. 2024-07-26T10:52:52Z 2024-07-26T10:52:52Z 2024-06-27 info:eu-repo/semantics/article Mallada, B. et. al. J. Phys. Chem. Lett. 2024, 15, 6935−6942. [https://doi.org/10.1021/acs.jpclett.4c00164] https://hdl.handle.net/10481/93522 10.1021/acs.jpclett.4c00164 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional ACS Publications