Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using Speed-Oligo MTBC Lara-Oya, Ana Rodríguez-Granger, Javier Moisés Liébana-Martos, María Del Carmen Mendoza-López, Pablo Sampedro-Martínez, Antonio Cobo, Fernando Gutiérrez Fernández, José Martínez-Lirola, Miguel José Navarro-Marí, José María We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosissensu stricto, 7 (3.7 %) as Mycobacteriumbovis, 5 (2.6 %) as M. bovis bacillus Calmette-Guérin, 2 (1.1 %) as Mycobacteriumafricanum and 1 (0.5 %) as Mycobacteriumcaprae; no strains belonged to Mycobacteriummicroti and Mycobacteriumcanettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment. 2024-07-26T07:23:42Z 2024-07-26T07:23:42Z 2016-09 journal article Lara-Oya A, Rodríguez-Granger JM, Liébana-Martos MDC, Mendoza-López P, Sampedro-Martínez A, Cobo F, Gutierrez-Fernández J, Martínez-Lirola MJ, Navarro-Marí JM. Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using Speed-Oligo MTBC. J Med Microbiol. 2016 Sep;65(9):910-914. https://hdl.handle.net/10481/93494 10.1099/jmm.0.000308 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional J Med Microbiol .