Volatiles of symbiotic bacterial origin explain ectoparasitism and fledging success of hoopoes Mazorra Alonso, Mónica Peralta-Sánchez, Juan Manuel Martín-Vivaldi Martínez, Manuel Lorenzo Martínez Bueno, Manuel Nuñez Gómez, Rafael Soler, Juan José Animal odour Avian microbiota Carnus hemapterus Background Some parasites use olfactory cues to detect their hosts and, since bacterial symbionts are partially responsible for animal odours, they could influence host parasitism. By autoclaving nest materials of hoopoe (Upupa epops) nests before reproduction started, we explored the hypothetical links between host-associated bacteria, volatiles and parasitism. During the nestling stage, we (i) estimated the level of ectoparasitism by chewing lice (Suborder Mallophaga) in adult hoopoe females and by Carnus haemapterus flies in nestlings, and (ii) characterized microbial communities and volatile profiles of nest environments (nest material and nest cavity, respectively) and uropygial secretions. Results Experimental nests had less diverse bacterial communities and more diverse volatile profiles than control nests, while occupants experienced lower intensity of parasitism in experimental than in control nests. The experiment also affected beta diversity of the microbial communities of nest material and of the volatiles of the nestling uropygial secretions. Moreover, microbial communities of uropygial secretions and of nest materials covaried with their volatile profiles, while the volatile profile of the bird secretions explained nest volatile profile. Finally, a subset of the volatiles and bacteria detected in the nest material and uropygial secretions were associated with the ectoparasitism intensity of both adult females and nestlings, and with fledging success. Conclusions These results show that a component of animal odours is linked with the microbial communities of the host and its reproductive environment, and emphasize that the associations between bacteria, ectoparasitism and reproductive success are partially mediated by volatiles of bacterial origin. Future work should focus on mechanisms underlying the detected patterns. 2024-07-24T10:16:50Z 2024-07-24T10:16:50Z 2024-05-10 journal article Mazorra‑Alonso et al. Animal Microbiome (2024) 6:26. [https://doi.org/10.1186/s42523-024-00312-9] https://hdl.handle.net/10481/93444 10.1186/s42523-024-00312-9 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional BMC