Emerging mycotoxin occurrence in chicken feed and eggs from Algeria Laouni, Chahinez Lara Vargas, Francisco Jesús Messai, Ahmed Redouane Salah, Sara Hernández Mesa, Maykel Gámiz Gracia, Laura García Campaña, Ana M Mycotoxins Poultry feed Eggs Poultry farming has developed into one of Algeria’s most productive industrial farming because of the growing demand for sources of protein among Algerian society. Laying hen feed consists mainly of cereals, which can be contaminated with molds and subsequently with their secondary metabolites known as mycotoxins. These later can pose a serious danger to the production and quality of eggs in the commercial layer industry. This work focuses on the detection of emerging mycotoxins, mainly enniatins (ENNs) and beauvericin (BEA), in poultry feed and eggs from different locations in Algeria. Two different QuEChERS-based extractions were established to extract ENNs and BEA from chicken feed and eggs. The determination of mycotoxin occurrence was achieved by a UHPLC-MS/MS method using 0.1% (v/v) formic acid in water and MeOH as mobile phase, an ESI interface operating in positive mode, and a triple quadrupole mass spectrometer operating in MRM for the detection. Matrix-matched calibration curves were carried out for both matrices, obtaining good linearity (R2 > 0.99). The method performance was assessed in terms of extraction recovery (from 87 to 107%), matrix effect (from − 47 to − 86%), precision (RSD < 15%), and limits of quantitation (≤ 1.1 μg/kg for feed and ≤ 0.8 μg/kg for eggs). The analysis of 10 chicken feed samples and 35 egg samples composed of a 10-egg pool each showed that ENN B1 was the most common mycotoxin (i.e., found in 9 feed samples) with contamination levels ranging from 3.6 to 41.5 μg/kg, while BEA was detected only in one feed sample (12 μg/kg). However, eggs were not found to be contaminated with any mycotoxin at the detection limit levels. Our findings indicate that the searched mycotoxins are present in traces in feed and absent in eggs. This can be explained by the application of a mycotoxin binder. However, this does not put a stop on the conduction of additional research and ultimately setting regulations to prevent the occurrence of emerging mycotoxins. 2024-07-24T08:00:49Z 2024-07-24T08:00:49Z 2024-05-16 journal article Laouni, C. et. al. Mycotoxin Res 40, 447–456 (2024). [https://doi.org/10.1007/s12550-024-00537-2] https://hdl.handle.net/10481/93429 10.1007/s12550-024-00537-2 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Springer