Encapsulation of Tenebrio molitor Hydrolysate with DPP-IV Inhibitory Activity by Electrospraying and Spray-Drying Berraquero García, Carmen Martínez-Sánchez, Lydia Guadix Escobar, Emilia María García Moreno, Pedro Jesús Antidiabetic peptides Enzymatic hydrolysis Nano-microencapsulation This study investigates the encapsulation of Tenebrio molitor hydrolysate exhibiting DPP-IV inhibitory activity by spray-drying and electrospraying techniques. First, we optimized the feed formulation and processing conditions required to obtain nano-microcapsules by electrospraying when using Arabic gum as an encapsulating agent and pullulan and Tween 20 as additives. The optimum formulation was also dried by spray-drying, where the removal of the additives was also assayed. Morphology analysis reveals that electrosprayed capsules have a smaller size (1.2 0.5 m vs. 12.4 8.7 m) and greater uniformity compared to those obtained by spray-drying. Regarding the surface nitrogen content and DPP-IV inhibitory activity, our results show no significant difference between the electrosprayed capsules and spray-dried capsules containing additives (IC50 of ~1.5 mg protein/mL). Therefore, it was concluded that adding additives during spray-drying allows for a similar encapsulation efficiency and reduced degradation during processing, as achieved by electrospraying technique but providing higher productivity. On the other hand, spray-dried capsules without additives displayed a higher surface nitrogen content percentage, which was mainly due to the absence of Tween 20 in the feed formulation. Consequently, these capsules presented a higher IC50 value (IC50 of 1.99 0.03 mg protein/mL) due to the potential degradation of surface-exposed peptides. 2024-07-23T11:36:07Z 2024-07-23T11:36:07Z 2024-05-10 journal article Berraquero García, C. et. al. Nanomaterials 2024, 14, 840. [https://doi.org/10.3390/nano14100840] https://hdl.handle.net/10481/93411 10.3390/nano14100840 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI