Assessment of Untargeted Metabolomics by Hydrophilic Interaction Liquid Chromatography−Mass Spectrometry to Define Breast Cancer Liquid Biopsy-Based Biomarkers in Plasma Samples González Olmedo, Carmen Díaz Beltrán, Leticia Madrid García, Verónica Palacios Ferrer, José Luis Cano Jiménez, Alicia Urbano Cubero, Rocío Pérez del Palacio, José Diaz, Caridad Vicente, Francisca Sanchez-Rovira, Pedro early diagnosis breast cancer mass spectrometry Abstract: An early diagnosis of cancer is fundamental not only in regard to reducing its mortality rate but also in terms of counteracting the progression of the tumor in the initial stages. Breast cancer (BC) is the most common tumor pathology in women and the second deathliest cancer worldwide, although its survival rate is increasing thanks to improvements in screening programs. However, the most common techniques to detect a breast tumor tend to be time-consuming, unspecific or invasive. Herein, the use of untargeted hydrophilic interaction liquid chromatography−mass spectrometry analysis appears as an analytical technique with potential use for the early detection of biomarkers in liquid biopsies from BC patients. In this research, plasma samples from 134 BC patients were compared with 136 from healthy controls (HC), and multivariate statistical analyses showed a clear separation between four BC phenotypes (LA, LB, HER2, and TN) and the HC group. As a result, we identified two candidate biomarkers that discriminated between the groups under study with a VIP > 1 and an AUC of 0.958. Thus, targeting the specific aberrant metabolic pathways in future studies may allow for better molecular stratification or early detection of the disease. 2024-07-22T08:20:42Z 2024-07-22T08:20:42Z 2024-05-07 journal article González Olmedo, C. et. al. Int. J. Mol. Sci. 2024, 25, 5098. [https://doi.org/10.3390/ ijms25105098] https://hdl.handle.net/10481/93334 10.3390/ ijms25105098 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI