Exercise dose on hepatic fat and cardiovascular health in adolescents with excess of adiposity González-Ruíz, K Correa-Bautista, JE Izquierdo, M García-Hermoso, A Martínez-Vizcaíno, V Lobelo, F González-Jiménez, E Schmidt Río Valle, Jacqueline Correa-Rodríguez, M Fernández-Irígoyen, J Palomino-Echeverría, S Santamaría, E Ramírez-Vélez, R Trial Registration clinicaltrials.gov Identifier: NCT02753231 We thank the HEPAFIT Study participants. Oriana Sarmiento, Ana Collazos, Daniel Prieto, Viviana Rincon, Juan Andrade, Lina Uribe, Liseth Yasno, and Victor Angulo, who contributed to study coordination, exercise training, and evaluation of study participants. Alejandra Tordecilla and Alejandra Hernandez assisted with training and provided ongoing advice on body composition analysis. Rosa Helena Bustos and Maria A. Dominguez collaborated throughout the trial. Additionally, we would like to thank the official school (Colegio Instituto Técnico Internacional) and teacher participants, as well as the master’s in physical activity and health programme and exercise trainers/students specialized from USTA and CEMA-UR. Objective: The HEPAFIT study was aimed at examining the impact of a 6-month physical education intervention, considering various levels of exercise intensity, on hepatic fat and cardiometabolic health outcomes in adolescents with excess adiposity. Methods: Adolescents (11 to 17 years with excess adiposity by body fat >30%), were randomly assigned to one of the following 4 groups for 6 months: (1) standard physical education lessons, control (CTRL); (2) high-intensity physical education(HIPE); (3) low-to-moderate intensity physical education(LIPE); (4) combined HIPE and LIPE(PLUS). The primary outcome was hepatic fat content measured by vibration-controlled transient elastography (controlled attenuation parameter [CAP]). Secondary outcomes were traditional cardiovascular health markers (body composition, serum lipids, aminotransferases, and health-related physical fitness components). Results: Adjusted mixed effects linear models revealed a significant decrease in CAP levels in HIPE (−13.81 dB/m, p=0.030) (p=0.001 vs CTRL group) and PLUS (−22.41 dB/m, p=0.001) (p=0.002 vs CTRL group) groups. Body fat decreased in the HIPE, −1.47 %, p<0.001) and PLUS, −1.88 %, p=0.001) groups. The physical fitness components were increased in the HIPE and PLUS group relative to the baseline (p<0.05), and the HIPE group showed a reduction in the total cholesterol (p<0.05). Conclusions: Implementation of a 6-month physical education exercise program, particularly high-intensity or combined high and low-intensity, improves hepatic fat storage and significantly reduces cardiometabolic markers in adolescents with excess of adiposity. Interventions involving supervised physical exercise may help to improve metabolism and fat deposition at the hepatic level, thus preventing the development of non-alcoholic fatty liver disease in adolescents. Trial Registration clinicaltrials.gov Identifier: NCT02753231 2024-07-22T06:53:36Z 2024-07-22T06:53:36Z 2022-04 journal article González-Ruíz K, Correa-Bautista JE, Izquierdo M, García-Hermoso A, Martínez-Vizcaíno V, Lobelo F, González-Jiménez E, Schmidt-RioValle J, Correa-Rodríguez M, Fernández-Irigoyen J, Palomino-Echeverría S, Santamaría E, Ramírez-Vélez R. Exercise dose on hepatic fat and cardiovascular health in adolescents with excess of adiposity. Pediatr Obes. 2022 Apr;17(4):e12869. doi: 10.1111/ijpo.12869. Epub 2021 Nov 4. PMID: 34734674. https://hdl.handle.net/10481/93296 10.1111/ijpo.12869 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional